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克隆相关的、体细胞发生突变的杂交瘤细胞中未表达的免疫球蛋白重链基因座的序列分析。

Sequence analysis of non-expressed immunoglobulin heavy chain loci in clonally related, somatically mutated hybridoma cells.

作者信息

Sablitzky F, Weisbaum D, Rajewsky K

出版信息

EMBO J. 1985 Dec 16;4(13A):3435-7. doi: 10.1002/j.1460-2075.1985.tb04101.x.

DOI:10.1002/j.1460-2075.1985.tb04101.x
PMID:3937726
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554681/
Abstract

The non-expressed, rearranged JH loci of two hybridoma cells, thought to be derived from a single B cell precursor were cloned and partially sequenced. Identical DJ rearrangements including N sequences at the DJ border were found, proving the common origin of the cells. The non-expressed loci lacked a rearranged VH gene and exhibited a single point mutation in a stretch of 1108 bp. This contrasts with 11 somatic point mutations in the 702 bp of the expressed VDJ regions. We discuss the possibility that the mechanism of somatic hypermutation may require V gene rearrangement.

摘要

对两个杂交瘤细胞的未表达重排JH基因座进行了克隆和部分测序，这两个杂交瘤细胞被认为源自单个B细胞前体。发现了相同的DJ重排，包括DJ边界处的N序列，证明了这些细胞的共同起源。未表达的基因座缺乏重排的VH基因，并且在一段1108 bp的序列中表现出一个单点突变。这与表达的VDJ区域702 bp中的11个体细胞点突变形成对比。我们讨论了体细胞超突变机制可能需要V基因重排的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a72/554681/39b58500ac41/emboj00278-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a72/554681/a7de65af9075/emboj00278-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a72/554681/39b58500ac41/emboj00278-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a72/554681/a7de65af9075/emboj00278-0076-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0a72/554681/39b58500ac41/emboj00278-0077-a.jpg

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本文引用的文献

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Somatic mutations of immunoglobulin variable genes are restricted to the rearranged V gene.免疫球蛋白可变基因的体细胞突变仅限于重排的V基因。
Science. 1983 Jun 10;220(4602):1179-81. doi: 10.1126/science.6857243.
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免疫球蛋白重链连接区基因序列及其在抗体多样性产生中的作用。
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4
Somatic diversification of immunoglobulins.免疫球蛋白的体细胞多样化
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5
Joining of immunoglobulin heavy chain gene segments: implications from a chromosome with evidence of three D-JH fusions.免疫球蛋白重链基因片段的连接：来自一条有三个D-JH融合证据的染色体的启示
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6
Differences between germ-line and rearranged immunoglobulin V kappa coding sequences suggest a localized mutation mechanism.种系免疫球蛋白Vκ编码序列与重排免疫球蛋白Vκ编码序列之间的差异提示了一种局部突变机制。
Nature. 1981 Jun 25;291(5817):668-70. doi: 10.1038/291668a0.
7
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.利用单链噬菌体克隆辅助快速DNA测序。
J Mol Biol. 1980 Oct 25;143(2):161-78. doi: 10.1016/0022-2836(80)90196-5.
8
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