Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Cangzhou Medical College, Cangzhou, Hebei 061001, China.
Int J Biol Macromol. 2024 Nov;281(Pt 4):136541. doi: 10.1016/j.ijbiomac.2024.136541. Epub 2024 Oct 12.
The development of functional dressings based on natural polysaccharide-based hydrogels remains a great challenge, and the specific roles of gel composition and drug-controlled release mechanisms were unclear. In this study, oxidized soybean cellulose nanocrystals (CNCs)/poly-acrylamide (PAM) hydrogel was prepared. The proportion of CNCs, crosslinkers, and water in the system was regulated to fine-tune the rheological performances, texture properties, transparency, and micro-network structures of CNCs/PAM hydrogel, and further explored its application potential in the field of wound dressings. It was found that CNCs improved the rigidity and adhesion of hydrogels, crosslinkers improved the network density, and water promoted the softening and fluidity of hydrogels. The effective filling of CNCs in the composite hydrogel was verified by FTIR, XRD, and NMR. Furthermore, the pH responsiveness and drug-loading potential of the smart hydrogel were tested by swelling and drug-controlled release experiments, elucidating drug-release dynamic mechanisms during the wound healing process. The inhibition zones (>7 mm) of gram-positive/negative bacteria and cell viability (>100 %) assay showed satisfactory biocompatibility, as the hydrogel effectively accelerated wound healing in a wound model. These results elucidated the regulation mechanism of the structures of CNCs/PAM hydrogel and revealed the application potential of CNCs/PAM hydrogel in wound dressings.
基于天然多糖水凝胶的功能性敷料的发展仍然是一个巨大的挑战，凝胶组成和药物控制释放机制的具体作用尚不清楚。在本研究中，制备了氧化大豆纤维素纳米晶（CNCs）/聚丙烯酰胺（PAM）水凝胶。通过调节 CNCs、交联剂和水在体系中的比例，精细调控了 CNCs/PAM 水凝胶的流变性能、质构特性、透明度和微网络结构，并进一步探索了其在伤口敷料领域的应用潜力。结果发现，CNCs 提高了水凝胶的刚性和附着力，交联剂提高了网络密度，而水则促进了水凝胶的软化和流动性。FTIR、XRD 和 NMR 验证了复合水凝胶中 CNCs 的有效填充。此外，还通过溶胀和药物控制释放实验测试了智能水凝胶的 pH 响应性和载药潜力，阐明了在伤口愈合过程中药物释放的动态机制。对革兰氏阳性/阴性菌的抑制区（>7mm）和细胞活力（>100%）测定表明，水凝胶具有良好的生物相容性，可有效促进伤口愈合。这些结果阐明了 CNCs/PAM 水凝胶结构的调节机制，并揭示了 CNCs/PAM 水凝胶在伤口敷料中的应用潜力。
