Su Yujie, Cai Yundan, Chang Cuihua, Li Junhua, Sun Yuanyuan, Zhao Qianwen, Huang Zijian, Xiong Wen, Gu Luping, Yang Yanjun
State Key Laboratory of Food Science and Resources, Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi, 214122 Jiangsu China.
College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128 Hunan China.
J Food Sci Technol. 2024 Nov;61(11):2111-2120. doi: 10.1007/s13197-024-05981-7. Epub 2024 Apr 24.
Egg yolk lipids are important components in egg yolk. Compared with proteins, minerals, vitamins and pigments in egg yolk, there is a lack of systematic research on extraction methods and characteristics of yolk lipid. Therefore, in this study, the extraction of egg yolk lipids by supercritical CO fluid extraction (SFE), subcritical propane extraction (SPE) and ethanol solvent extraction (SE) was studied, and the differences in composition and physicochemical properties of the three yolk lipids were analyzed. The egg yolk lipids extracted by SFE had the advantages of high unsaturated fatty acid content, low saponification value, acid value, peroxide value, and high content of neutral lipids such as triglyceride and cholesterol, but the polar phospholipids were completely retained in the yolk powder. The efficiency of lipid extraction of SPE was the highest among all the three methods, but the extraction capacity to the polar phospholipid was in the middle of the three methods. The efficiency of lipid extraction of SE was low, but the extraction capacity to the polar phospholipid was the highest. Suitable extraction methods could be selected based on the specific application requirements of egg yolk lipids and low-fat egg yolk powders.
蛋黄脂质是蛋黄中的重要成分。与蛋黄中的蛋白质、矿物质、维生素和色素相比,关于蛋黄脂质提取方法及其特性的系统研究较少。因此,本研究对超临界CO₂流体萃取(SFE)、亚临界丙烷萃取(SPE)和乙醇溶剂萃取(SE)提取蛋黄脂质的方法进行了研究,并分析了三种蛋黄脂质在组成和理化性质上的差异。超临界CO₂流体萃取法提取的蛋黄脂质具有不饱和脂肪酸含量高、皂化值、酸值、过氧化值低,以及甘油三酯和胆固醇等中性脂质含量高的优点,但极性磷脂完全保留在蛋黄粉中。三种方法中,亚临界丙烷萃取法的脂质提取效率最高,但对极性磷脂的提取能力处于三种方法的中间水平。乙醇溶剂萃取法的脂质提取效率较低,但对极性磷脂的提取能力最高。可根据蛋黄脂质和低脂蛋黄粉的具体应用需求选择合适的提取方法。