Department of Dermatology and Venereology, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, 413 45 Gothenburg, Sweden.
Faculty of Medicine, Sarajevo School of Science and Technology (SSST), 71000 Sarajevo, Bosnia and Herzegovina.
Int J Mol Sci. 2024 Sep 24;25(19):10239. doi: 10.3390/ijms251910239.
The gut microbiota plays a critical role in immune system function, with dysbiosis linked to systemic inflammation, contributing to conditions like psoriasis and depression. Although biological treatments for severe psoriasis are known to impact gut bacteria, less is understood about their effects on fungi. This study aims to investigate fungal gut microbiota changes in psoriasis patients transitioning from TNF-α inhibitors to brodalumab. Fecal samples from 20 patients were analyzed using Illumina MiSeq sequencing of the ITS2 region of 18S rRNA. Microbial diversity was assessed through Bray-Curtis dissimilarity and the Shannon-Wiener index. Clinical outcomes were measured using clinical scores for psoriasis and depression severity, with statistical analysis performed via Wilcoxon signed-rank tests and PERMANOVA. Results showed that Ascomycota was the dominant fungal phylum in both treatment groups, with , , , and as prevalent genera. No significant changes occurred at the phylum level after switching to brodalumab, though minor genome-level variations were observed. Beta diversity analysis highlighted inter-patient variability, with no significant correlation between fungal composition and clinical outcomes. Despite improved clinical scores, the fungal gut microbiota remained largely stable, suggesting that brodalumab does not significantly alter fungal communities in psoriasis patients. Further research is needed for a comprehensive understanding.
肠道微生物群在免疫系统功能中起着关键作用，其失调与全身炎症有关，导致银屑病和抑郁症等疾病的发生。虽然已知生物疗法可影响重度银屑病患者的肠道细菌，但对其真菌的影响了解较少。本研究旨在调查从 TNF-α 抑制剂转换为布罗达卢单抗的银屑病患者肠道真菌微生物群的变化。使用 Illumina MiSeq 对 18S rRNA 的 ITS2 区进行测序，分析了 20 名患者的粪便样本。通过 Bray-Curtis 不相似性和 Shannon-Wiener 指数评估微生物多样性。使用银屑病和抑郁严重程度的临床评分来衡量临床结果，通过 Wilcoxon 符号秩检验和 PERMANOVA 进行统计分析。结果表明，在治疗组中，子囊菌门是主要的真菌门，其中 、 、 和 是常见的属。尽管转换为布罗达卢单抗后，在门水平上没有发生显著变化，但观察到了微小的基因组水平变化。β多样性分析突出了患者间的变异性，真菌组成与临床结果之间没有显著相关性。尽管临床评分有所改善，但肠道真菌微生物群仍基本稳定，表明布罗达卢单抗不会显著改变银屑病患者的真菌群落。需要进一步研究以全面了解。
