Alhaiqi Nawal S, Afifi Sherine M, Mahyoub Jazem A, Abdel-Gaber Rewaida A, Delic Denis, Dkhil Mohamed A
Department of Zoology, Faculty of Women for Arts, Sciences and Education - Ain Shams University; Egypt.
Department of Biological Sciences, Faculty of Science, King Abdul Aziz University, Jeddah, Saudi Arabia.
Comb Chem High Throughput Screen. 2025;28(8):1310-1319. doi: 10.2174/0113862073341577240925100048.
Helminthiasis remains a major global health concern. Exploring natural alternatives due to drug resistance and synthetic drug side effects has become increasingly urgent.
This study investigates the anthelmintic potential of leaf extracts (CPLE) against , along with elucidating the underlying structural alterations and molecular interactions. Carica papaya underwent methanolic extraction. Gas chromatography- mass spectrometry analysis revealed 11 active phytochemical compounds within CPLE. The anthelmintic activity was evaluated against A. caliginosa, with CPLE demonstrating efficacy comparable to albendazole. Light microscopy and scanning electron microscopy depicted structural modifications in worms exposed to CPLE, characterized by reduced size, uniform shrinkage, and increased cuticle thickness.
Molecular docking studies with proteins β-tubulin and Trichuris trichiura β-tubulin revealed potential binding interactions of CPLE compounds, notably Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, and Albendazole oxide. Conclusión: These findings suggest the anthelmintic efficacy of CPLE and provide insights into its mode of action at the molecular level.
蠕虫病仍然是全球主要的健康问题。由于耐药性和合成药物副作用，探索天然替代物变得越来越迫切。
本研究调查了番木瓜叶提取物（CPLE）对[具体寄生虫名称缺失]的驱虫潜力，并阐明其潜在的结构改变和分子相互作用。对番木瓜进行了甲醇提取。气相色谱-质谱分析显示CPLE中含有11种活性植物化学化合物。针对微小隐孢子虫评估了其驱虫活性，CPLE显示出与阿苯达唑相当的疗效。光学显微镜和扫描电子显微镜描绘了暴露于CPLE的蠕虫的结构变化，其特征为尺寸减小、均匀收缩和角质层厚度增加。
与蛋白质β-微管蛋白和 Trichuris trichiura β-微管蛋白的分子对接研究揭示了CPLE化合物的潜在结合相互作用，特别是十六烷酸、2-羟基-1-（羟甲基）乙酯和阿苯达唑亚砜。结论：这些发现表明CPLE具有驱虫功效，并在分子水平上为其作用模式提供了见解。
