School of Pharmacy, Guizhou Medical University, Guiyang 550025, China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528400, China.
Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan 528400, China.
Chin J Nat Med. 2024 Oct;22(10):945-954. doi: 10.1016/S1875-5364(24)60722-3.
The phytochemical investigation of the leaves and twigs of Croton yunnanensis resulted in the isolation of eight new clerodane furanoditerpenoids, named croyunfuranoids A-H (1-8), along with three known analogs (9-11). The structures of these compounds were elucidated using spectroscopic analyses, and their absolute configurations were determined through a combination of electronic circular dichroism (ECD) calculations and single-crystal X-ray diffraction. Notably, Croyunfuranoid D (4) is identified as a rare 18,19-dinor-clerodane diterpenoid. Additionally, the structure of a previously reported diterpenoid, crotonyunnan B, was revised. All isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages. Among them, compounds 5 and 6 demonstrated significant inhibitory effects, with IC values of 20.33 ± 2.31 and 22.80 ± 1.31 μmol·L, respectively.
从云南算盘子的叶和枝中分离得到 8 个新的裂环烯醚萜呋喃二萜，命名为 croyunfuranoids A-H（1-8），同时还分离得到 3 个已知类似物（9-11）。通过光谱分析阐明了这些化合物的结构，并通过电子圆二色性（ECD）计算和单晶 X 射线衍射相结合确定了它们的绝对构型。值得注意的是，Croyunfuranoid D（4）被鉴定为罕见的 18,19-降克拉烷二萜。此外，还修正了先前报道的二萜化合物 crotonyunnan B 的结构。所有分离得到的化合物均对 LPS 诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）产生的抑制活性进行了评价。其中，化合物 5 和 6 表现出显著的抑制作用，IC 值分别为 20.33±2.31μmol·L 和 22.80±1.31μmol·L。
