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L-吡咯烷酮-β-萘酰胺水解试验在鉴定特定革兰氏阳性球菌中的价值

Value of the L-pyrrolidonyl-beta-naphthylamide hydrolysis test for identification of select gram-positive cocci.

作者信息

Oberhofer T R

出版信息

Diagn Microbiol Infect Dis. 1986 Jan;4(1):43-7. doi: 10.1016/0732-8893(86)90055-6.

DOI:10.1016/0732-8893(86)90055-6
PMID:3943290
Abstract

A 4 hr test for hydrolysis of L-pyrrolidonyl-beta-naphthylamide (PYR) was evaluated for its use in identifying group A beta-hemolytic streptococci, group D enterococci, and Staphylococcus haemolyticus. All of the group A streptococci and the enterococci tested were positive in the PYR test, whereas all of the nongroup A, B, or D beta-hemolytic streptococci, all of the viridans, and all of the Streptococcus pneumoniae isolates tested were PYR negative. Two strains of Aerococcus species were PYR positive and could not be differentiated from enterococci in this test. All strains of S. haemolyticus, a single strain of S. intermedius and two of seven strains of S. warneri were PYR positive whereas all other staphylococci tested were PYR negative. The PYR test is suitable for routine, presumptive identification of group A streptococci, group D enterococci and S. haemolyticus in the clinical laboratory.

摘要

对L-吡咯烷酮基-β-萘酰胺(PYR)水解的4小时试验进行了评估,以确定其在鉴定A群β溶血性链球菌、D群肠球菌和溶血葡萄球菌中的用途。所有测试的A群链球菌和肠球菌在PYR试验中均为阳性,而所有非A、B或D群β溶血性链球菌、所有草绿色链球菌和所有测试的肺炎链球菌分离株均为PYR阴性。两株气球菌属菌株PYR阳性,在此试验中无法与肠球菌区分开来。所有溶血葡萄球菌菌株、一株中间葡萄球菌菌株和七株沃氏葡萄球菌菌株中的两株PYR阳性,而所有其他测试的葡萄球菌均为PYR阴性。PYR试验适用于临床实验室对A群链球菌、D群肠球菌和溶血葡萄球菌进行常规的初步鉴定。

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