Synthesis, characterization and application of silk sericin-based silver nanocomposites for antibacterial and food coating solutions.

The rising demand for fresh and safe food is driving advancements in preservation technologies, with nanoparticles offering a revolutionary solution. These particles extend shelf life, preserve nutritional value, and enhance food safety, aligning with present consumer expectations. This study explores the eco-friendly synthesis, characterization, and application of silk sericin-based silver nanoparticles (SS-AgNPs) for antibacterial and food coating purposes. Silk sericin, a byproduct of the silk industry, is typically discarded despite its valuable properties like biocompatibility, biodegradability, and antimicrobial activity. In this research, sericin from cocoons was used as a reducing and stabilizing agent to synthesize SS-AgNPs. Characterization was performed using UV-vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and dynamic light scattering (DLS). Antibacterial tests confirmed the efficacy of SS-AgNPs against sp. and sp., while food coating trials on tomatoes significantly reduced weight loss and microbial contamination. Biocompatibility was further verified through hemolysis and MTT assays, confirming SS-AgNPs' safety for biomedical and food-related uses. This study underscores the potential to convert sericin waste into a valuable resource, promoting sustainability and increasing the commercial value of sericulture.