El-Zehery Hoda R A, Ashry Noha Mohamed, Faiesal Abeer A, Attia Mohamed S, Abdel-Maksoud Mostafa A, El-Tayeb Mohamed A, Aufy Mohammed, El-Dougdoug Noha K
Department of Agricultural Microbiology, Faculty of Agriculture, Benha University, Benha, Egypt.
Department of Basic and Applied Agricultural Sciences, Higher Institute for Agriculture Cooperation, Cairo, Egypt.
Front Microbiol. 2024 Oct 7;15:1448191. doi: 10.3389/fmicb.2024.1448191. eCollection 2024.
Fungal endophytes are known to produce bioactive chemicals and secondary metabolites that are often identical to those produced by their host plants. The main objective of the current study was to isolate and identify endophytic fungi associated with the medicinal plant , and to investigate their potential antibacterial and anticancer properties. The ethyl acetate extracts from the isolated endophytic fungi, as well as the host plant , were subjected to bioactivity assays to evaluate their antibacterial and anticancer potential against multi-drug resistant bacterial strains and the human hepatocellular carcinoma cell line HepG2. The endophytic fungi isolated and identified from the samples included and species. The minimum inhibitory concentration (MIC) assay revealed that the extract exhibited the strongest antibacterial activity, with an MIC value of 4 μg/ml, followed by the sp. (5 μg/ml) and sp. (6 μg/ml) extracts. Additionally, the crude extracts of sp., sp., and sp. demonstrated high anticancer activity against HepG2 cells, with inhibition rates ranging from 89 to 92% at a concentration of 50 μg/ml. Interestingly, the extract showed the most potent anticancer activity, with a 95% inhibition rate against HepG2 cells at the same concentration. These findings highlight the significant potential of endophytic fungi associated with , as a source of bioactive compounds with promising antibacterial and anticancer properties. The results reinforce the hypothesis that medicinal plants and their endophytic fungi can serve as an attractive alternative for the development of novel therapeutic agents, potentially offering a more sustainable and less harmful approach to disease management compared to traditional chemical-based methods.
已知真菌内生菌会产生生物活性化学物质和次生代谢产物，这些物质通常与它们的宿主植物所产生的物质相同。本研究的主要目的是分离和鉴定与该药用植物相关的内生真菌，并研究它们潜在的抗菌和抗癌特性。对分离出的内生真菌以及宿主植物的乙酸乙酯提取物进行生物活性测定，以评估它们对多重耐药细菌菌株和人肝癌细胞系HepG2的抗菌和抗癌潜力。从样本中分离和鉴定出的内生真菌包括 和 物种。最低抑菌浓度（MIC）测定表明， 提取物表现出最强的抗菌活性，MIC值为4μg/ml，其次是 属（5μg/ml）和 属（6μg/ml）提取物。此外， 属、 属和 属的粗提物对HepG2细胞表现出高抗癌活性，在浓度为50μg/ml时抑制率为89%至92%。有趣的是， 提取物表现出最有效的抗癌活性，在相同浓度下对HepG2细胞抑制率为95%。这些发现突出了与 相关的内生真菌作为具有前景的抗菌和抗癌特性生物活性化合物来源的巨大潜力。结果强化了这样一种假设，即药用植物及其内生真菌可以作为开发新型治疗剂的有吸引力的替代方案，与传统的基于化学的方法相比，可能为疾病管理提供一种更可持续、危害更小的方法。
