Babu Krishna Moorthy, Bentall Daniel, Ashton David T, Puklowski Morgan, Fantham Warren, Lin Harris T, Tuckey Nicholas P L, Wellenreuther Maren, Jesson Linley K
The New Zealand Institute for Plant and Food Research Limited, Lincoln, New Zealand.
The New Zealand Institute for Plant and Food Research Limited, Nelson, New Zealand.
J R Soc N Z. 2022 Aug 3;53(1):52-68. doi: 10.1080/03036758.2022.2101484. eCollection 2023.
In aquaculture breeding or production programmes, counting juvenile fish represents a considerable cost in terms of the human hours needed. In this study, we explored the use of two state-of-the-art machine learning architectures (Single Shot Detection, hereafter SSD and Faster Regions with convolutional neural networks, hereafter Faster R-CNN) to augment a manual image-based juvenile fish counting method for the Australasian snapper () bred at The New Zealand Institute for Plant and Food Research Limited. We tested model accuracy after tuning for confidence thresholds and non-maximal suppression overlap parameters, and implementing a bias correction using a Poisson regression model. Validation of image data showed that after tuning, bias-corrected SSD and Faster R-CNN models had mean absolute percent errors (MAPE) of less than 10%, with SSD having MAPE of less than 5%. Comparison of the results with those from manual counts showed that, while manual counts are slightly more accurate (MAPE = 1.56), the machine learning methods allow for more rapid assessment of counts and thus facilitating a higher throughput. This work represents a first step for deploying machine learning applications to an existing real-life aquaculture scenario and provides a useful starting point for further developments, such as real-time counting of fish or collecting additional phenotypic data from the source images.
在水产养殖育种或生产计划中,对幼鱼进行计数在所需人工时长方面会产生相当大的成本。在本研究中,我们探索了使用两种最先进的机器学习架构(单发检测器,以下简称SSD和带有卷积神经网络的更快区域,以下简称Faster R-CNN)来改进一种基于图像的人工幼鱼计数方法,该方法用于新西兰植物与食品研究所有限公司养殖的新西兰红鱼()。我们在调整置信度阈值和非极大值抑制重叠参数后测试了模型准确性,并使用泊松回归模型进行偏差校正。图像数据验证表明,调整后,经偏差校正的SSD和Faster R-CNN模型的平均绝对百分比误差(MAPE)小于10%,其中SSD的MAPE小于5%。将结果与人工计数结果进行比较表明,虽然人工计数略为准确(MAPE = 1.56),但机器学习方法能够更快速地评估计数,从而实现更高的通量。这项工作代表了将机器学习应用部署到现有实际水产养殖场景的第一步,并为进一步的发展提供了一个有用的起点,例如鱼类的实时计数或从源图像中收集额外的表型数据。