Teagasc Food Research Centre, Moorepark, Cork, Ireland.
APC Microbiome Ireland, Cork, Ireland.
Int J Syst Evol Microbiol. 2024 Oct;74(10). doi: 10.1099/ijsem.0.006549.
Four strains, representing two novel species, were isolated from water kefir, a fermented beverage. 16S rRNA gene analysis suggested that the novel species share high identities (98.82-98.89%) with LMG 28769. Complete genomes were assembled with a short- and long-read hybrid sequencing approach. In agreement with the 16S rRNA gene analysis, phylogenetics with 117 marker genes places the novel species closest to LMG 28769 as well. The isolates have average nucleotide identity (ANI) scores ranging from 81.46 to 84.84% and digital DNA-DNA hybridization (dDDH) scores from 23.9 to 38.5% with the closest related species, as well as ANI scores between the proposed new species of 80.50%, indicating that the isolates represent two novel species. Matrix-assisted laser desorption/ionization-time of flight chemotaxonomic analysis supported the gene-based taxonomic placement. We propose the names sp. nov. and sp. nov. for these novel species within the genus. The proposed type strain WK012_4_13 (= LMG 33104 = DSM 116073; GenBank accession number GCF_041080835.1) has a genome size of 2.43 Mbp, with a G+C content of 56.00 mol%. The proposed type strain for WK041_4_12 (= LMG 33105 = DSM 116074; GenBank accession number GCF_041080795.1) has a genome size of 2.36 Mbp and a G+C content of 53.94 mol%. cells are Gram-positive staining, non-motile, non-spore-forming, fructose-6-phosphate phosphoketolase (F6PPK)-positive, catalase- and oxidase-negative and bacillary club shaped. cells are Gram-positive staining, non-motile, non-spore-forming, F6PPK-positive, catalase- and oxidase-negative and square rod shaped.
从发酵饮料——水开菲尔中分离到的 4 株菌，代表了两个新的种。16S rRNA 基因分析表明，这两个新种与 LMG 28769 的相似度很高（98.82-98.89%）。通过短读长和长读长混合测序方法对全基因组进行组装。与 16S rRNA 基因分析一致，基于 117 个标记基因的系统发育分析也将这两个新种与 LMG 28769 放在最接近的位置。这些分离株的平均核苷酸同一性（ANI）得分在 81.46%到 84.84%之间，与最接近的相关种的数字 DNA-DNA 杂交（dDDH）得分在 23.9%到 38.5%之间，与所提出的新种的 ANI 得分在 80.50%之间，表明这些分离株代表了两个新种。基质辅助激光解吸/电离飞行时间化学分类分析支持基于基因的分类定位。我们建议将这些新种分别命名为 sp. nov. 和 sp. nov.。提议的模式菌株 WK012_4_13（=LMG 33104=DSM 116073；GenBank 登录号 GCF_041080835.1）的基因组大小为 2.43 Mbp，GC 含量为 56.00 mol%。提议的模式菌株 WK041_4_12（=LMG 33105=DSM 116074；GenBank 登录号 GCF_041080795.1）的基因组大小为 2.36 Mbp，GC 含量为 53.94 mol%。WK012_4_13 细胞革兰氏阳性染色，非运动，不产孢，果糖-6-磷酸磷酸酮醇酶（F6PPK）阳性，过氧化氢酶和氧化酶阴性，棒状。WK041_4_12 细胞革兰氏阳性染色，非运动，不产孢，F6PPK 阳性，过氧化氢酶和氧化酶阴性，方形杆状。
