Chen Yahan, Lu Yunshuang, Dong Shibo, Yang Chengde, Yang Shunyi
College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China.
Plants (Basel). 2024 Oct 18;13(20):2924. doi: 10.3390/plants13202924.
The autophagy-related gene family (Autophagy-related 8) plays an important role in plant growth, development, and stress response. In this study, 15 gene family sequences were amplified from Solanaceae, namely tobacco, tomato, and pepper, using RT-PCR to evaluate their basic properties, protein structure, and function, as well as the role of in autophagy. The physicochemical properties, the predicted secondary and tertiary protein structures, subcellular localisation, gene structures, conserved motifs, and phylogenetic relationships of the genes were analysed using bioinformatic techniques, and their expression patterns under sericin-induced plant disease resistance were investigated by RT-qPCR. The lengths of these proteins ranged from 79 to 120 aa, while their predicted molecular weights and isoelectric points () ranged from 9283.62 to 13,778.74 and 6.32 to 11.44, respectively. The majority of the proteins were localised in the nucleus or chloroplasts. Conserved protein motifs and various cis-regulatory elements in the protein, with a wide range of related functions, were identified. The gene family members showed expression changes after treatment with osthole, which induces disease resistance in tobacco, tomato, and pepper. These findings provide a foundation for further analyses of the gene family in Solanaceae and the mechanism underlying the response to adverse conditions.
自噬相关基因家族（自噬相关8）在植物生长、发育和应激反应中发挥着重要作用。在本研究中，利用RT-PCR从茄科植物烟草、番茄和辣椒中扩增出15个该基因家族序列，以评估它们的基本特性、蛋白质结构和功能，以及在自噬中的作用。使用生物信息学技术分析了这些基因的理化性质、预测的蛋白质二级和三级结构、亚细胞定位、基因结构、保守基序和系统发育关系，并通过RT-qPCR研究了它们在丝胶诱导的植物抗病性下的表达模式。这些蛋白质的长度在79至120个氨基酸之间，而其预测的分子量和等电点分别在9283.62至13778.74和6.32至11.44之间。大多数蛋白质定位于细胞核或叶绿体中。鉴定出了蛋白质中的保守基序和各种具有广泛相关功能的顺式调控元件。在用蛇床子素处理后，该基因家族成员表现出表达变化，蛇床子素可诱导烟草、番茄和辣椒的抗病性。这些发现为进一步分析茄科植物中的该基因家族以及对逆境响应的潜在机制奠定了基础。
