Graduate School of Life and Health Sciences, Chubu University, 1200 Matsumoto-cho, Kasugai-shi 487-8501, Aichi, Japan.
Support for Pioneering Research Initiated by the Next Generation (SPRING), Chubu University, 1200 Matsumoto-cho, Kasugai-shi 487-8501, Japan.
Viruses. 2024 Oct 19;16(10):1636. doi: 10.3390/v16101636.
Influenza A virus (IAV) infection contributes to high annual morbidity and mortality, thus necessitating measures aimed at protecting against the disease. Alcohol-based disinfectants are commonly used to inactivate IAV, but they have several undesirable properties. In search of other means which would inactivate IAV, we focused on the effect of alkaline solutions on IAV. We found the viral infectivity remarkably decreased with treatment of an alkaline solution at pH 12.0 for 1 min, where destruction of the viral spikes was observed using an electron microscope. A more detailed examination revealed that the infectivity of IAV was remarkedly reduced by brief treatment with the alkaline solution at pH 11.75 or above, most likely due to the degradation of viral hemagglutinin protein. These results show that at a high pH, the haemagglutinin protein is degraded, resulting in very rapid inactivation of IAV.
甲型流感病毒（IAV）感染会导致高发病率和死亡率，因此需要采取措施预防疾病。含酒精的消毒剂常用于灭活 IAV，但它们有几个不理想的特性。为了寻找其他灭活 IAV 的方法，我们专注于碱性溶液对 IAV 的影响。我们发现，当 pH 值为 12.0 的碱性溶液处理 1 分钟时，病毒的感染力显著下降，用电子显微镜观察到病毒刺突被破坏。更详细的检查表明，IAV 的感染力在用 pH 值为 11.75 或更高的碱性溶液短暂处理后显著降低，这很可能是由于病毒血凝素蛋白的降解。这些结果表明，在高 pH 值下，血凝素蛋白被降解，导致 IAV 非常迅速地失活。
