Department of Anatomical Pathology, Edo State University, Uzairue, Edo State, Nigeria.
Department of Pathology, University College Hospital, Ibadan, Oyo State, Nigeria.
Pan Afr Med J. 2024 Jul 7;48:90. doi: 10.11604/pamj.2024.48.90.42773. eCollection 2024.
cervical cancer is primarily driven by high-risk human papillomavirus infections. It is a leading cause of cancer-related deaths among women globally. The emergence of immunotherapeutic approaches, particularly programmed death ligand-1 (PD-L1) inhibitors, has shown promise in various cancers. This study aims to investigate the correlation between PD-L1 expression and HPV status in cervical carcinoma samples from a Nigerian tertiary hospital.
the study was conducted in the Department of Pathology of our hospital. The study materials were 101 cases of archival formalin-fixed paraffin-embedded (FFPE) tissue blocks that met the study criteria recruited retrospectively from January 2012 to December 2016. Immunohistochemistry for PD-L1 was done and real time PCR for HPV DNA was done using CFX96. The data were then analyzed using SPSS version 23. P < 0.05 was considered significant.
high-risk HPV detection rate was 51%. The two most common genotypes were HPV 16 (84.3%) and HPV 35 (17.6%). The predominant infections were single genotypes occurring in 80.4% of the cases. There was no correlation of HPV status with PD-L1, histological grade or type of cervical carcinoma. High-risk HPV did not show any distribution pattern with age groups of patients.
human papillomavirus 16 is the most predominant cause of cervical carcinoma. There are some environmental variations in the frequency of other genotypes with HPV 35 being relatively more common than HPV 18 in this study. Programmed death ligand-1 was positive in 47% of the cases but did not show any correlation with the HPV infection status.
宫颈癌主要由高危型人乳头瘤病毒（HPV）感染引起。它是全球女性癌症相关死亡的主要原因。免疫治疗方法，特别是程序性死亡配体 1（PD-L1）抑制剂，在各种癌症中显示出了希望。本研究旨在调查尼日利亚一家三级医院宫颈癌样本中 PD-L1 表达与 HPV 状态之间的相关性。
该研究在我们医院的病理学系进行。研究材料为 101 例符合研究标准的存档福尔马林固定石蜡包埋（FFPE）组织块，这些组织块是从 2012 年 1 月至 2016 年 12 月回顾性招募的。对 PD-L1 进行免疫组织化学染色，使用 CFX96 进行 HPV DNA 的实时 PCR。然后使用 SPSS 版本 23 对数据进行分析。P < 0.05 被认为具有统计学意义。
高危型 HPV 检出率为 51%。最常见的两种基因型是 HPV 16（84.3%）和 HPV 35（17.6%）。主要感染是单一基因型，占 80.4%的病例。HPV 状态与 PD-L1、组织学分级或宫颈癌类型之间没有相关性。高危型 HPV 与患者年龄组之间没有分布模式。
HPV 16 是宫颈癌的最主要原因。其他基因型的频率存在一些环境差异，本研究中 HPV 35 相对 HPV 18 更为常见。PD-L1 在 47%的病例中呈阳性，但与 HPV 感染状态没有相关性。
