中国中部和东部地区 2306 例宫颈鳞癌患者的 HPV 流行率和基因型分布。
HPV prevalence and genotype distribution in 2,306 patients with cervical squamous cell carcinoma in central and eastern China.
机构信息
Department of Pathology, Nanjing Lishui People's Hospital (Zhongda Hospital Lishui Branch), Southeast University, Nanjing, China.
Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
出版信息
Front Public Health. 2023 Aug 28;11:1225652. doi: 10.3389/fpubh.2023.1225652. eCollection 2023.
BACKGROUND
To explore the positivity rate and genotype distribution of human papillomavirus (HPV) in cervical squamous cell carcinoma (CSCC) tissues in central and eastern China and to provide theoretical basis for cervical cancer screening and prophylactic HPV vaccine development in China.
METHODS
DNA was extracted from paraffin-embedded tissues of CSCC samples and exfoliated cervical cells of cervical cancer screening populations. 23 HPV genotypes were detected by combining polymerase chain reaction (PCR) and reverse dot hybridized gene chip detection technology in 2,306 CSCC tissues and 10,245 cervical cancer screening populations. The genotype distribution of HPV infection was analyzed.
RESULTS
The overall infection rate of HPVs in 2,306 CSCC patients was 92.71%. The frequency of single-type HPV infection and multiple-type HPV infection were 86.48% and 13.51%, respectively. The most common HPV genotypes detected in Chinese CSCC tissues were HPV-16, HPV-18, HPV-31, HPV-33, HPV-45, HPV-52, HPV-58, and HPV-59. The overall positivity rate of these eight high-risk HPV (HR-HPV) genotypes in HPV-positive CSCC was as high as 96.91%. Of which the positivity rate of seven HR-HPV genotypes related to nine-valent HPV vaccines in HPV-positive CSCC was 95.09%. Meanwhile, the overall infection rates of HR-HPV and low-risk HPV (LR-HPV) in female aged 35-64 years who underwent cervical cancer screening were 13.16% and 1.32%, respectively. The high-frequency HR-HPV genotypes in cervical cancer screening women were HPV-52, HPV-58, HPV-16, HPV-53, HPV-68, HPV-39, HPV-51, and HPV-56, with positivity rates of 2.25%, 1.60%, 1.31%, 1.22%, 0.93%, 0.92%, 0.78%, and 0.74%, respectively.
CONCLUSION
Among women screened for cervical cancer in China, detecting the 8 high-frequency HR-HPV genotypes can reduce technical difficulty and reagent costs, while also improving the efficiency and effectiveness of cervical cancer screening. HPV genotyping assists gynecologists in assessing the risk of HR-HPV-positive cervical intraepithelial neoplasia and guiding them in implementing appropriate interventions. Furthermore, HPV genotyping is helpful for doctors to follow up HR-HPV-positive women and to evaluate the protective effect of HPV vaccine.
背景
探讨中国中部和东部地区宫颈鳞状细胞癌（CSCC）组织中人乳头瘤病毒（HPV）的阳性率和基因型分布，为我国宫颈癌筛查和预防性 HPV 疫苗的开发提供理论依据。
方法
采用聚合酶链反应（PCR）结合反向斑点杂交基因芯片检测技术，从 2306 例 CSCC 组织和 10245 例宫颈癌筛查人群的石蜡包埋组织和宫颈脱落细胞中提取 DNA，分析 HPV 感染的基因型分布。
结果
2306 例 CSCC 患者 HPV 总感染率为 92.71%。单一型 HPV 感染和多型 HPV 感染的频率分别为 86.48%和 13.51%。在中国 CSCC 组织中检测到的最常见 HPV 基因型为 HPV-16、HPV-18、HPV-31、HPV-33、HPV-45、HPV-52、HPV-58 和 HPV-59。HPV 阳性 CSCC 中这八种高危 HPV（HR-HPV）基因型的总阳性率高达 96.91%。其中 HPV 阳性 CSCC 中与九价 HPV 疫苗相关的七种 HR-HPV 基因型的阳性率为 95.09%。同时，35-64 岁行宫颈癌筛查女性 HR-HPV 和低危型 HPV（LR-HPV）的总感染率分别为 13.16%和 1.32%。宫颈癌筛查女性中高频 HR-HPV 基因型为 HPV-52、HPV-58、HPV-16、HPV-53、HPV-68、HPV-39、HPV-51 和 HPV-56，阳性率分别为 2.25%、1.60%、1.31%、1.22%、0.93%、0.92%、0.78%和 0.74%。
结论
在中国宫颈癌筛查人群中，检测 8 种高频 HR-HPV 基因型可以降低技术难度和试剂成本，同时提高宫颈癌筛查的效率和效果。HPV 基因分型有助于妇科医生评估 HR-HPV 阳性宫颈上皮内瘤变的风险，并指导实施适当的干预措施。此外，HPV 基因分型有助于医生对 HR-HPV 阳性女性进行随访，并评估 HPV 疫苗的保护效果。
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