Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, P. R. China.
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, P. R. China.
Sci Data. 2024 Oct 29;11(1):1171. doi: 10.1038/s41597-024-04028-y.
Megachile is one of the largest bee genera, including nearly 1,500 species, but very few chromosome-level assemblies exist for this group or the family Megachilidae. Here, we report the chromosome-level genome assembly of Megachile lagopoda collected from Xizang, China. Using PacBio CLR long reads and Hi-C data, we assembled a genome of 256.83 Mb with 96.08% of the assembly located on 16 chromosomes. Our assembly contains 266 scaffolds, with a scaffold N50 length of 15.6 Mb, and BUSCO completeness of 99.20%. We masked 27.10% (69.61 Mb) of the assembly as repetitive elements, identified 459 non-coding RNAs, and predicted 11,157 protein-coding genes. This high-quality genome of M. lagopoda represents an important step forward for our knowledge of megachilid genomics and bee evolution overall.
巨蜂是最大的蜜蜂属之一，包含近 1500 个物种，但该属或 Megachilidae 科的染色体水平基因组组装非常少。在这里，我们报告了来自中国西藏的 Megachile lagopoda 的染色体水平基因组组装。使用 PacBio CLR 长读段和 Hi-C 数据，我们组装了一个 256.83Mb 的基因组，其中 96.08%的组装位于 16 条染色体上。我们的组装包含 266 个支架，支架 N50 长度为 15.6Mb，BUSCO 完整性为 99.20%。我们将 27.10%（69.61Mb）的组装掩蔽为重复元件，鉴定了 459 个非编码 RNA，并预测了 11157 个蛋白质编码基因。M. lagopoda 的这个高质量基因组代表了我们对 megachilid 基因组学和总体蜜蜂进化的认识的重要一步。
