大木蜂（Xylocopa dejeanii Lepeletier，1841年）（膜翅目：蜜蜂科）的染色体水平基因组组装

Chromosome-level genome assembly of the large carpenter bee Xylocopa dejeanii Lepeletier, 1841 (Hymenoptera: Apidae).

作者信息

Zhang Dan, Jin Jianfeng, Niu Zeqing, Orr Michael C, Zhang Feng, Ferrari Rafael R, Wu Qingtao, Zhou Qingsong, Da Wa, Zhu Chaodong, Luo Arong

机构信息

State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, P.R. China.

Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, P.R. China.

出版信息

Sci Data. 2025 Jul 23;12(1):1280. doi: 10.1038/s41597-025-05641-1.

DOI:10.1038/s41597-025-05641-1

PMID:40702009

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12287282/

Abstract

Xylocopinae, a diverse bee subfamily comprising over 1,000 bee species, and also a major model system for studying the pollination and evolution of sociality. The lack of chromosome-level genome assembly resources for the Xylocopinae limits our research of their biology and evolution. Here, we provided the first pseudo-chromosomes genome assembly of the Xylocopa dejeanii combined PacBio CLR long reads, Illumina sequences, and Hi-C data. The final genome is 194.44 Mb located in 16 chromosomes. Our assembly includes 141 scaffolds, with a scaffold N50 length of 13.15 Mb. BUSCO analysis revealed 99.00% completeness. Genome annotation identified 28.27 Mb of repetitive elements, 10,970 protein-coding genes, and 432 ncRNAs. This high-quality X. dejeanii assembly advances our understanding of Xylocopinae genomics and provides new insights into bee evolution.

摘要

木蜂亚科是一个多样化的蜜蜂亚科，包含1000多种蜜蜂，也是研究授粉和社会性进化的主要模式系统。木蜂亚科缺乏染色体水平的基因组组装资源，这限制了我们对其生物学和进化的研究。在这里，我们结合PacBio CLR长读长、Illumina序列和Hi-C数据，首次提供了德氏木蜂的假染色体基因组组装。最终的基因组大小为194.44 Mb，分布在16条染色体上。我们的组装包括141个支架，支架N50长度为13.15 Mb。BUSCO分析显示完整性为99.00%。基因组注释鉴定出28.27 Mb的重复元件、109