Institute of Physics, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Division of Science and Technology, Department of Physics, University of Education, Lahore, Pakistan.
Sci Rep. 2024 Nov 2;14(1):26398. doi: 10.1038/s41598-024-78320-7.
Several bacterial strains have developed resistance against commercial antibiotics, and interestingly, supramolecular nanomaterials have shown considerable advantages for antibacterial applications. However, the main challenges in adopting nanotechnology for antibacterial studies are random aggregation, compromised toxicity, multi-step preparation approaches, and unclear structure-function properties. Herein, we designed the amphiphilic tripeptide that acts as a reducing and capping agent for silver metal to form silver-peptide colloidal nanohybrids with the mild assistance of UV light (254 nm) through the photochemical reduction method. The nanohybrids are characterized by different spectroscopic and microscopic techniques, and non-covalent molecular interactions between metal and peptide building blocks confirm their central role in the formation of nanohybrids. The tripeptide is biocompatible and can reduce the toxicity of silver ions (Ag) by reducing to Ag. These colloidal nanohybrids showed antibacterial activity against gram-negative and gram-positive bacterial strains, and the possible mechanism of killing bacterial cells could be membrane disruption. This synthetic strategy is facile and green, which helps avoid using toxic chemicals or reagents and complicated methods for colloidal nanohybrid preparation for biomedical applications.
几种细菌菌株已经对商业抗生素产生了耐药性，有趣的是，超分子纳米材料在抗菌应用方面显示出了相当大的优势。然而，将纳米技术应用于抗菌研究的主要挑战是随机聚集、毒性降低、多步制备方法以及结构-功能性质不明确。在本文中，我们设计了一种两亲三肽，它可以作为银金属的还原剂和封端剂，通过光化学还原法在 254nm 紫外光的温和辅助下形成银-肽胶体纳米杂化材料。纳米杂化材料通过不同的光谱和显微镜技术进行了表征，金属和肽构建块之间的非共价分子相互作用证实了它们在纳米杂化材料形成中的核心作用。三肽具有生物相容性，可以通过还原 Ag 来降低银离子 (Ag) 的毒性。这些胶体纳米杂化材料对革兰氏阴性和革兰氏阳性细菌菌株表现出抗菌活性，杀死细菌细胞的可能机制是破坏细胞膜。这种合成策略简单、绿色，有助于避免在胶体纳米杂化材料的制备中使用有毒化学物质或试剂以及复杂的方法，从而可将其应用于生物医学领域。
