Sistemich Linda, Ebbinghaus Simon
Chair of Biophysical Chemistry, Ruhr-University Bochum, Germany.
Research Center Chemical Sciences and Sustainability, Research Alliance Ruhr, Bochum, Germany.
FEBS Open Bio. 2024 Dec;14(12):1940-1954. doi: 10.1002/2211-5463.13912. Epub 2024 Nov 3.
Thermal heating of biological samples allows to reversibly manipulate cellular processes with high temporal and spatial resolution. Manifold heating techniques in combination with live-cell imaging were developed, commonly tailored to customized applications. They include Peltier elements and microfluidics for homogenous sample heating as well as infrared lasers and radiation absorption by nanostructures for spot heating. A prerequisite of all techniques is that the induced temperature changes are measured precisely which can be the main challenge considering subcellular structures or multicellular organisms as target regions. This article discusses heating and temperature sensing techniques for live-cell imaging, leading to future applications in cell biology.
对生物样品进行热加热能够以高时空分辨率对细胞过程进行可逆操作。人们开发了多种加热技术并与活细胞成像相结合，这些技术通常是针对定制应用而量身定制的。它们包括用于均匀样品加热的珀耳帖元件和微流体技术，以及用于点加热的红外激光和纳米结构的辐射吸收技术。所有技术的一个先决条件是精确测量诱导的温度变化，考虑到以亚细胞结构或多细胞生物作为目标区域，这可能是主要挑战。本文讨论了用于活细胞成像的加热和温度传感技术，这些技术将在细胞生物学领域带来未来的应用。
