National Center of Biotechnology (CSIC), Darwin 3, Campus Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Acta Crystallogr D Struct Biol. 2021 Jun 1;77(Pt 6):835-839. doi: 10.1107/S2059798321002291. Epub 2021 May 19.
Principal component analysis (PCA) has been widely proposed to analyze flexibility and heterogeneity in cryo-electron microscopy (cryoEM). In this paper, it is argued that (i) PCA is an excellent technique to describe continuous flexibility at low resolution (but not so much at high resolution) and (ii) PCA components should be analyzed in a concerted manner (and not independently).
主成分分析（PCA）已被广泛应用于分析低温电子显微镜（cryoEM）中的灵活性和异质性。本文认为：（i）PCA 是描述低分辨率下连续灵活性的绝佳技术（但在高分辨率下则不然）；（ii）应协同分析 PCA 成分（而不是独立分析）。
