Navarro A S, Lanao J M, Dominguez-Gil Hurlé A
J Antimicrob Chemother. 1986 Jan;17(1):83-9. doi: 10.1093/jac/17.1.83.
The in-vitro inactivation kinetics of dibekacin by three semi-synthetic penicillins (carbenicillin, ampicillin and ticarcillin) were studied taking into account the influence of certain factors such as time, the concentration of the semi-synthetic penicillin in the sample and the reaction medium used in the inactivation process. For a dibekacin/beta lactam concentration ratio of 1/100, the maximum percentage of inactivation of dibekacin was obtained for ticarcillin (99%), followed by carbenicillin (90%) and finally by ampicillin (65%) over a period of 45 h. For carbenicillin and ampicillin a linear relationship was established between the inactivation constant (Ki) and the concentration of these penicillins in the sample. It was also found that under the same experimental conditions the inactivation percentage in human serum was smaller than that observed in buffered solution, pH = 7.4. It was observed that the degree of inactivation of dibekacin and gentamicin was similar under the experimental conditions employed in this study.
研究了三种半合成青霉素（羧苄西林、氨苄西林和替卡西林）对双去甲氧基卡那霉素的体外失活动力学，同时考虑了某些因素的影响，如时间、样品中半合成青霉素的浓度以及失活过程中使用的反应介质。当双去甲氧基卡那霉素与β-内酰胺的浓度比为1/100时，在45小时内，替卡西林对双去甲氧基卡那霉素的失活百分比最高（99%），其次是羧苄西林（90%），最后是氨苄西林（65%）。对于羧苄西林和氨苄西林，失活常数（Ki）与样品中这些青霉素的浓度之间建立了线性关系。还发现，在相同的实验条件下，人血清中的失活百分比低于pH = 7.4的缓冲溶液中的失活百分比。在本研究采用的实验条件下，观察到双去甲氧基卡那霉素和庆大霉素的失活程度相似。
