Gong Huxuan, Tan Xiao, Hou Jiaojiao, Gong Zan, Qin Xiangxiang, Nie Jing, Zhu Hua, Zhong Shian
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China.
Guangxi University of Chinese Medicine, Nanning 530200, China.
Int J Biol Macromol. 2024 Dec;282(Pt 6):137232. doi: 10.1016/j.ijbiomac.2024.137232. Epub 2024 Nov 8.
In this study, ACP-1, a water-soluble polysaccharide was isolated from the roots of Alocasia cucullata (AC) using freeze-thaw treatment (FTT). Structural characterization revealed ACP-1 (2.10×10 Da) to be a homogeneous heteropolysaccharide primarily consisting of glucose, galactose, and arabinose, and a trace of fucose, rhamnose, and glucuronic acid. Methylation analysis and nuclear magnetic resonance spectroscopy revealed that the backbone of ACP-1 consisted of →[3)-β-D-Galp-(1]→3,6)-β-D-Glcp-(1→3,6)-β-D-Glcp-(1→, with a branch at C-3. In vitro experiments demonstrated that ACP-1 significantly enhanced the proliferation and phagocytosis of RAW264.7 cells, upregulated the expression of co-stimulatory molecules (CD80 and CD86), and activated RAW264.7 cells via the nuclear factor kappa-B signal transduction pathway, resulting in nitric monoxide release and the secretion of the cytokines tumor necrosis factor-α, interleukin (IL)-1β, and IL-6. Overall, ACP-1 shows potential as a novel immunostimulant.
在本研究中，采用冻融处理（FTT）从尖尾芋（AC）的根中分离出一种水溶性多糖ACP-1。结构表征显示，ACP-1（2.10×10 Da）是一种均一的杂多糖，主要由葡萄糖、半乳糖和阿拉伯糖组成，并含有微量的岩藻糖、鼠李糖和葡萄糖醛酸。甲基化分析和核磁共振光谱表明，ACP-1的主链由→[3)-β-D-半乳糖-(1]→3,6)-β-D-葡萄糖-(1→3,6)-β-D-葡萄糖-(1→组成，在C-3处有一个分支。体外实验表明，ACP-1显著增强了RAW264.7细胞的增殖和吞噬作用，上调了共刺激分子（CD80和CD86）的表达，并通过核因子κB信号转导途径激活RAW264.7细胞，导致一氧化氮释放以及细胞因子肿瘤坏死因子-α、白细胞介素（IL）-1β和IL-6的分泌。总体而言，ACP-1作为一种新型免疫刺激剂具有潜力。
