A Mathesh, Snega Ramanathan, Geetha Sravanthy P, Saravanan Muthupandian
Department of Pharmacology, Antimicrobial Resistance (AMR) and Nanotherapeutics Lab, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, IND.
Cureus. 2024 Oct 14;16(10):e71502. doi: 10.7759/cureus.71502. eCollection 2024 Oct.
a type of cyanobacterium (blue-green algae), is well known for its rich abundant nutritional profile and bioactive compounds, which contribute to various biological functions within the human body. The application of nanotechnology to Spirulina has the potential to further enhance its biological activity in biomedical assays.
This study aimed to utilize for the green synthesis of cerium oxide nanoparticles (CeO-NPs) and evaluate their physiochemical properties. The research will assess the antibacterial and anti-inflammatory efficacy of the synthesized nanoparticles and explore the underlying mechanisms of action.
-mediatedcerium oxide nanoparticlesare synthesized by the green synthesis (titration method). The biosynthesized CeO-NPs are characterized by using techniques such as UV-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy, X-ray diffraction spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray (EDX). Antibacterial activity was carried out by the agar-well diffusion method and anti-inflammatory activity was carried out by the albumin denaturation method.
The green synthesis of cerium oxide nanoparticles (CeO-NPs) using Spirulina, a sustainable and eco-friendly method has potential application in antibacterial and anti-inflammatory therapies. This study focuses on the green synthesis of CeO-NPs and characterizes them by using UV-Vis, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), SEM, and EDX. The UV-vis analysis confirmed the presence of CeO-NPs at a wavelength of 320 nm. FT-IR reveals four functional groups, such as C-O, N-O, and C=C stretches. XRD analysis showed higher crystalline and less amorphous content. SEM and EDX spectra were utilized to confirm the morphology (agglomerated square shape) and the elemental composition [Ce, O, C] in the CeO-NPs. The antibacterial activity was evaluated against multidrug-resistant (MDR) clinical strains and the anti-inflammatory activity revealed significant activity in a dose-dependent manner.
This study concluded that Spirulina-mediated CeO-NPs have potential as a drug in biomedical assays. Further in vitro and in vivo analysis is required to fully confirm their viability as a potential drug.
螺旋藻是一种蓝藻（蓝绿藻），以其丰富的营养成分和生物活性化合物而闻名，这些成分有助于人体的各种生物学功能。将纳米技术应用于螺旋藻有可能在生物医学检测中进一步增强其生物活性。
本研究旨在利用螺旋藻进行绿色合成氧化铈纳米颗粒（CeO-NPs）并评估其理化性质。该研究将评估合成纳米颗粒的抗菌和抗炎功效，并探索其潜在的作用机制。
通过绿色合成法（滴定法）合成螺旋藻介导的氧化铈纳米颗粒。利用紫外可见光谱（UV-Vis）、傅里叶变换红外光谱、X射线衍射光谱、扫描电子显微镜（SEM）和能量色散X射线（EDX）等技术对生物合成的CeO-NPs进行表征。通过琼脂孔扩散法进行抗菌活性检测，通过白蛋白变性法进行抗炎活性检测。
利用螺旋藻绿色合成氧化铈纳米颗粒（CeO-NPs），这一可持续且环保的方法在抗菌和抗炎治疗中具有潜在应用价值。本研究聚焦于CeO-NPs的绿色合成，并通过UV-Vis、傅里叶变换红外光谱（FT-IR）、X射线衍射分析（XRD）、SEM和EDX对其进行表征。紫外可见分析证实了在320nm波长处存在CeO-NPs。FT-IR揭示了四个官能团，如C-O、N-O和C=C伸缩振动。XRD分析显示结晶度较高且无定形含量较少。利用SEM和EDX光谱确认了CeO-NPs的形态（团聚方形）和元素组成[Ce、O、C]。对多药耐药（MDR）临床菌株进行了抗菌活性评估，抗炎活性显示出显著的剂量依赖性活性。
本研究得出结论，螺旋藻介导的CeO-NPs在生物医学检测中具有作为药物的潜力。需要进一步的体外和体内分析来充分证实它们作为潜在药物的可行性。
