Department of Pathology and Microbiology, College of Veterinary Medicine, University of Duhok, Duhok, Iraq.
Department of Veterinary Public Health, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Open Vet J. 2024 Sep;14(9):2256-2260. doi: 10.5455/OVJ.2024.v14.i9.13. Epub 2024 Sep 30.
has emerged as a major public health concern. It is a common pathogen in animal and human medicine.
Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) was used to fingerprint 10 strains of obtained from nasal swabs in domesticated animals and humans to ascertain how comparable the different strains' genetic makeup was.
These isolates were previously identified using standard molecular and microbiological methods. ERIC primers were amplified for all isolates. The dendrogram was generated using PGMA and the dice similarity coefficient. The strains were genotyped according to the diversity of sample source (human or animal), and the geographic source.
Ten strains were classified into eight "ERIC" kinds (genotypes) using "ERIC-PCR genotyping", in which the two most common clones were genotypes 8 and 2, which were represented by one strain from humans, one from cows, and two strains from sheep. Two strains derived from separate geographic areas and from different sample sources (human and cow) were determined to share the same genotype. Another two strains from different geographic areas but from the same sample source (sheep) were categorized under the same genotype. All the remaining strains were classified as a singular genotype.
This study supports the possible bacterial transmission from animal to human and from animals themselves that usually happens during live animal marketing. Recognizing the interconnected nature of transmission systems and implementing the required approaches to disease prevention and control is essential for mitigating the risks posed by bacterial pathogens.
已成为一个主要的公共卫生关注点。它是动物和人类医学中的常见病原体。
使用肠杆菌重复基因间一致性聚合酶链反应（ERIC-PCR）对 10 株从家养动物和人类鼻拭子中获得的 进行基因指纹图谱分析，以确定不同菌株的遗传组成有多么相似。
这些分离株先前使用标准分子和微生物学方法进行了鉴定。对所有分离株进行 ERIC 引物扩增。使用 PGMA 和骰子相似系数生成聚类图。根据样本来源（人类或动物）和地理来源对菌株进行基因分型。
使用“ERIC-PCR 基因分型”将 10 株 分为 8 种“ERIC”类型（基因型），其中最常见的两个克隆是基因型 8 和 2，它们分别由 1 株来自人类、1 株来自牛和 2 株来自绵羊的菌株代表。来自不同地理区域和不同样本来源（人类和牛）的两个菌株被确定具有相同的基因型。来自不同地理区域但来自同一样本来源（绵羊）的另外两个菌株被归类为同一基因型。其余所有菌株均被归类为单一基因型。
本研究支持细菌从动物向人类传播以及动物之间传播的可能性，这种传播通常发生在活体动物市场期间。认识到传播系统的相互关联性质并实施必要的疾病预防和控制方法对于减轻细菌病原体带来的风险至关重要。
