Miyauchi K, Ogawa M, Shibata T, Matsuda K, Mori T, Ito K, Minamiura N, Yamamoto T
Clin Chim Acta. 1986 Jan 30;154(2):115-23. doi: 10.1016/0009-8981(86)90003-3.
A reliable radioimmunoassay (RIA) for human deoxyribonuclease I (DNase I) is described. Using delayed addition of tracer antigen, the method is sensitive (9.5 reproducible and specific. A good parallel relationship was observed between the standard curve and dilution curves for human urine and human pancreatic juice. G-actin, a naturally occurring DNase I inhibitor, caused no change in the immunoreactivity of DNase I. In healthy individuals, aged 11-90 yr, the mean serum DNase I was 18.4 ng/ml (SD 6.7 ng/ml). Increased serum DNase I occurred in patients with acute pancreatitis, renal failure, and in about one-third of patients with various malignant tumors.
本文描述了一种可靠的人脱氧核糖核酸酶I（DNase I）放射免疫分析方法。通过延迟加入示踪抗原，该方法灵敏（检测限为9.5可重复且特异。在人尿和人胰液的标准曲线与稀释曲线之间观察到良好的平行关系。G-肌动蛋白是一种天然存在的DNase I抑制剂，对DNase I的免疫反应性无影响。在11至90岁的健康个体中，血清DNase I的平均值为18.4 ng/ml（标准差6.7 ng/ml）。急性胰腺炎、肾衰竭患者以及约三分之一的各种恶性肿瘤患者血清DNase I升高。
