National Public Health Laboratory, 09 BP 24, 09, Ouagadougou, Burkina Faso.
Applied Biochemistry and Immunology Laboratory (LaBIA), Sciences and Technologies Doctoral School, KI-ZERBO University, 03 BP 7021 03, 03, Ouagadougou, Burkina Faso.
Food Environ Virol. 2024 Nov 24;17(1):2. doi: 10.1007/s12560-024-09621-3.
Raw vegetables irrigated with polluted water that may contain enteric viruses can be associated with foodborne viral disease outbreaks. The objective of this study is to investigate the possible transmission of enteric viruses from irrigation water to lettuce. Therefore, we performed a commercial multiplex real-time PCR assay to monitor the occurrence of enteric viruses in irrigation water samples and in raw vegetables that were cultivated at market gardening sites in Ouagadougou, Burkina Faso. Samples were collected from six market gardening sites located in Ouagadougou. RT-PCR was performed to detect norovirus GI, norovirus GII, rotavirus, enteric adenoviruses F (Serotype 40/41), astrovirus and sapovirus (Genogroups G1, 2, 4, 5). From the 10 irrigation water samples and the 80 lettuce samples, three (30%) and twenty-two (27.5%) were positive for enteric viruses, respectively. Norovirus GII, astrovirus and enteric adenoviruses F (Serotype 40/41) were the most frequently detected viruses in lettuce and irrigation water samples. Our results indicate that raw vegetables may be contaminated with a broad range of enteric viruses, which may originate from virus-contaminated irrigation water, and these vegetables may act as a potential vector of food-borne viral transmission.
用可能含有肠道病毒的污染水灌溉的生蔬菜可能与食源性病毒病暴发有关。本研究的目的是调查肠道病毒是否可能从灌溉水传播到生菜。因此，我们进行了商业多重实时 PCR 检测，以监测布基纳法索瓦加杜古市场园艺场灌溉水中和生蔬菜中肠道病毒的发生情况。从瓦加杜古的六个市场园艺场收集了样本。进行 RT-PCR 以检测诺如病毒 GI、诺如病毒 GII、轮状病毒、肠道腺病毒 F（血清型 40/41）、星状病毒和肠病毒（基因群 G1、2、4、5）。从 10 个灌溉水样和 80 个生菜样本中，分别有 3 个（30%）和 22 个（27.5%）样本检测到肠道病毒呈阳性。诺如病毒 GII、星状病毒和肠道腺病毒 F（血清型 40/41）是生菜和灌溉水样本中最常检测到的病毒。我们的结果表明，生蔬菜可能受到广泛的肠道病毒污染，这些病毒可能来自受病毒污染的灌溉水，这些蔬菜可能成为食源性病毒传播的潜在载体。
