Nemeth P M, Solanki L, Gordon D A, Hamm T M, Reinking R M, Stuart D G
J Neurosci. 1986 Mar;6(3):892-8. doi: 10.1523/JNEUROSCI.06-03-00892.1986.
Individual muscle fibers of 10 motor units from the tibialis posterior muscle of cat were identified by glycogen depletion techniques, characterized for histochemical type, diameter, and intramuscular locations, and analyzed by quantitative biochemical methods. Four enzymes, representing different energy-yielding pathways, were quantitatively assayed in muscle fibers belonging to motor units selected from each of the three major physiological types. All four enzymes demonstrated identical activities among fibers within a motor unit, while showing up to 11-fold differences among fibers belonging to different motor units. Moreover, fibers within a single motor unit, but of substantially different diameters, were nevertheless homogeneous in specific enzyme activities.
通过糖原耗竭技术鉴定了猫胫骨后肌10个运动单位的单个肌纤维,对其组织化学类型、直径和肌内位置进行了表征,并采用定量生化方法进行了分析。在从三种主要生理类型中选出的运动单位所属的肌纤维中,对代表不同能量产生途径的四种酶进行了定量测定。所有四种酶在一个运动单位内的纤维之间表现出相同的活性,而在属于不同运动单位的纤维之间则表现出高达11倍的差异。此外,单个运动单位内直径差异很大的纤维,其特定酶活性却是均匀一致的。