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CRISPR-Cas9 介导的 OsHPPD 3'UTR 编辑赋予水稻对 HPPD 抑制剂类除草剂的增强抗性。

CRISPR-Cas9-mediated editing of the OsHPPD 3' UTR confers enhanced resistance to HPPD-inhibiting herbicides in rice.

机构信息

Institute of Agricultural Sciences for Lixiahe Region in Jiangsu, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, China.

Institute of Agricultural Sciences for Lixiahe Region in Jiangsu, Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Yangzhou 225009, China.

出版信息

Plant Commun. 2023 Sep 11;4(5):100605. doi: 10.1016/j.xplc.2023.100605. Epub 2023 Apr 23.

DOI:10.1016/j.xplc.2023.100605
PMID:37087571
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10504582/
Abstract

This study reports the creation of herbicide-resistant rice lines via CRISPR-Cas9-mediated editing of the 3' UTR of OsHPPD. Resistance index calculations revealed that two resistant lines, TS8-2-10 and TS8-8-6, exhibited 4.8-fold and 3.7-fold greater resistance to HPPD-inhibiting herbicides compared with the wild type, YG3012.

摘要

本研究通过 CRISPR-Cas9 介导的 OsHPPD 3'UTR 编辑，创建了抗除草剂的水稻品系。抗性指数计算表明，与野生型 YG3012 相比，两个抗性品系 TS8-2-10 和 TS8-8-6 对 HPPD 抑制剂除草剂的抗性分别提高了 4.8 倍和 3.7 倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0551/10504582/e94f3bc28cf9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0551/10504582/e94f3bc28cf9/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0551/10504582/e94f3bc28cf9/gr1.jpg

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