Zhai Yi-Jie, Zhao Shu-Hui, Gao Jin-Ming, Han Wen-Bo
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China; College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.
Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Chemistry & Pharmacy, Northwest A&F University, Yangling 712100, Shaanxi, China.
Phytochemistry. 2025 Mar;231:114340. doi: 10.1016/j.phytochem.2024.114340. Epub 2024 Nov 26.
Four undescribed cytochalasins (1-4), three undescribed orsellinic acid derivatives (5-7) and two known metabolites including methyl lecanorate (8) and methyl orsellinate (9) were isolated from the solid-state cultivation of a soil-derived fungus Trichocladium asperum SQ2-3 collected in Qinghai-Tibet Plateau. Their structures were elucidated by analysis of NMR (1D and 2D) and mass spectrometry data. The absolute configurations of 1-7 were assigned by a combination of the modified Mosher's method, microscale derivatization and Mo(OAc)-induced circular dichroism experiment. Compounds 1, 2, 3 and 6 showed significant cytotoxicity against HL-60, A3494, SMMC-7721, MDA-MB-231 and SW480 cell lines with IC values ranging from 4.74 to 15.84 μM, respectively. Meanwhile, compound 1 could obviously damage mitochondrial membrane potential and induce G2/M cell cycle arrest in A549 cells.
从青藏高原采集的土壤来源真菌粗糙枝霉（Trichocladium asperum）SQ2 - 3的固态培养物中分离得到了4种未描述的细胞松弛素（1 - 4）、3种未描述的苔色酸衍生物（5 - 7）以及2种已知代谢产物，包括落叶松萝酸甲酯（8）和苔色酸甲酯（9）。通过对核磁共振（一维和二维）及质谱数据的分析阐明了它们的结构。通过改良的莫舍尔法、微量衍生化和钼（乙酸盐）诱导圆二色性实验相结合的方法确定了1 - 7的绝对构型。化合物1、2、3和6对HL - 60、A3494、SMMC - 7721、MDA - MB - 231和SW480细胞系显示出显著的细胞毒性，IC值分别为4.74至15.84 μM。同时，化合物1可明显破坏A549细胞的线粒体膜电位并诱导G2/M期细胞周期阻滞。
