Sessink P J, Friemèl N S, Anzion R B, Bos R P
Department of Toxicology, Faculty of Medical Sciences, University of Nijmegen, The Netherlands.
Int Arch Occup Environ Health. 1994;65(6):401-3. doi: 10.1007/BF00383251.
The exposure of 11 pharmaceutical plant workers to methotrexate (MTX) was studied. Personal air samples were taken during the different manufacturing processes: drug compounding, vial filling, and tablet preparation. The uptake of MTX was established by the determination of MTX in urine. MTX was analyzed using the fluorescence polarization immunoassay (FPIA), a method that is frequently used for monitoring serum levels in patients treated with MTX. The FPIA method was modified in such a way that MTX could be measured quickly and efficiently in air and urine samples. MTX was detected in air samples of all workers except for those involved in the vial filling process (range: 0.8-182 micrograms/m3; median: 10 micrograms/m3). The highest concentrations were observed for workers weighing MTX (118 and 182 micrograms/m3). MTX was detected in urine samples of all workers. The mean cumulative MTX excretion over 72-96 h was 13.4 micrograms MTX-equivalents (range: 6.1-24 micrograms MTX-equivalents). A significantly lower background level of 10.2 micrograms MTX-equivalents was measured in urine of 30 control persons (range: 4.9-21 micrograms MTX-equivalents).
对11名制药厂工人接触甲氨蝶呤（MTX）的情况进行了研究。在不同的生产过程中采集了个人空气样本：药物配制、小瓶灌装和片剂制备。通过测定尿液中的MTX来确定其摄入量。使用荧光偏振免疫分析法（FPIA）对MTX进行分析，该方法常用于监测接受MTX治疗患者的血清水平。对FPIA方法进行了改进，以便能够快速有效地测定空气和尿液样本中的MTX。除参与小瓶灌装过程的工人外，所有工人的空气样本中均检测到MTX（范围：0.8 - 182微克/立方米；中位数：10微克/立方米）。称量MTX的工人检测到的浓度最高（118和182微克/立方米）。所有工人的尿液样本中均检测到MTX。72 - 96小时内MTX的平均累积排泄量为13.4微克MTX当量（范围：6.1 - 24微克MTX当量）。在30名对照人员的尿液中测得的背景水平显著较低，为10.2微克MTX当量（范围：4.9 - 21微克MTX当量）。
