奥罗普切病毒的综合监测：巴西亚马逊地区一次疫情期间潜在城市传播媒介的分子证据

Integrated surveillance for Oropouche Virus: Molecular evidence of potential urban vectors during an outbreak in the Brazilian Amazon.

作者信息

Feitoza Luiz Henrique Maciel, Gasparelo Nadson Willian Felipe, Meireles Anne Caroline Alves, Rios Flávia Geovana Fontineles, Teixeira Karolaine Santos, da Silva Michelli Santos, Paz Matheus de Araújo, Roca Tárcio Peixoto, Moreira Hillquias Monteiro, de França Kamila Pereira, Dall'Acqua Deusilene Souza Vieira, Julião Genimar Rebouças, de Medeiros Jansen Fernandes

机构信息

Fiocruz Rondônia - Fundação Oswaldo Cruz, Laboratório de Entomologia, Porto Velho, Rondônia, Brazil; Programa de Pós-Graduação em Biologia Experimental - PGBIOEXP, Fundação Universidade Federal de Rondônia/ Fiocruz Rondônia, Porto Velho, Rondônia, Brazil.

Programa de Pós-Graduação em Biologia Experimental - PGBIOEXP, Fundação Universidade Federal de Rondônia/ Fiocruz Rondônia, Porto Velho, Rondônia, Brazil; Centro de Pesquisa em Medicina Tropical, Porto Velho, Rondônia, Brazil; Fiocruz Rondônia - Fundação Oswaldo Cruz, Laboratório de Virologia Molecular, Porto Velho, Rondônia, Brazil.

出版信息

Acta Trop. 2025 Jan;261:107487. doi: 10.1016/j.actatropica.2024.107487. Epub 2024 Dec 1.

DOI:10.1016/j.actatropica.2024.107487

PMID:39622307

Abstract

The Oropouche Virus (OROV) has Culicoides paraensis as its main vector in the urban cycle. Diagnoses of acute febrile cases and entomological collections were performed between January and April 2024 in the urban setting of Porto Velho, a city located in the western Brazilian Amazon. A total of 904 human samples were evaluated using RT-qPCR, of which 328 were positive for OROV. In addition, 3,950 insects were captured and Culex quinquefasciatus was the most abundant and predominant species. Seven mosquito samples showed amplification for OROV from pools of Cx. quinquefasciatus, Limatus durhamii and Aedes albopictus. Due to the first record of OROV in the two latter species, our findings can support new hypotheses about potential or bridge vectors. In turn, the high density of Cx. quinquefasciatus reinforces the supposition of this species as the main urban vector in the Amazon in the absence of C. paraensis. Complementary studies of vector competence and capacity are necessary for confirmation.

摘要

奥罗普切病毒（OROV）在城市传播周期中的主要传播媒介是副库蠓。2024年1月至4月期间，在位于巴西亚马逊西部的波多韦柳市的城市环境中进行了急性发热病例诊断和昆虫学采集。共使用逆转录定量聚合酶链反应（RT-qPCR）评估了904份人类样本，其中328份OROV呈阳性。此外，捕获了3950只昆虫，致倦库蚊是数量最多且占主导地位的物种。七个蚊子样本显示从致倦库蚊、达勒姆边斑蚊和白纹伊蚊的混合样本中扩增出了OROV。由于后两个物种中首次记录到OROV，我们的研究结果可为有关潜在或桥梁传播媒介的新假设提供支持。反过来，致倦库蚊的高密度强化了在没有副库蠓的情况下该物种作为亚马逊地区主要城市传播媒介的推测。需要进行传播媒介能力和容量的补充研究以进行确认。