M Chamundeeswari, Kr Preethy
Associate Professor, Department of Biotechnology, St. Joseph's College of Engineering, OMR, Chennai, India.
Assistant Professor, Department of Biotechnology, Rajalakshmi Engineering College, Thandalam, Chennai, India.
Biotechnol Appl Biochem. 2025 Aug;72(4):865-882. doi: 10.1002/bab.2702. Epub 2024 Dec 2.
Metal-based nanoparticles have been extensively researched for their distinctive characteristics. Among them, zinc oxide nanoparticles have numerous applications in the field of biomedicine. The phytoextract of Ixora coccinea flowers was used in the synthesis of ZnO nanoparticles replacing the use of harmful reducing chemicals. In the current research, the carbonaceous material from biowaste of Setaria italica was used to synthesize graphene oxide (GO) by Improved Hummer's method. The synthesized GO was converted to reduced GO via green nanotechnology using phytoextract of Prosopis juliflora. The synthesis of reduced Graphene Oxide - Zinc Oxide Nanocomposite (rGO)-ZnO nanocomposite involves a simple, economical one-step magnetic stirring method. UV-visible spectroscopy was used to characterize the synthesized materials, with the maximal absorbance range for ZInc Oxide (ZnO) being 384 nm and for rGO-ZnO composite at 243 and 366 nm, respectively. The x-ray diffraction (XRD) revealed 2θ peaks for ZnO at 31.54°, 34.22°, and 36.08°. For reduced Graphene Oxide (rGO) in rGO-ZnO composite, the XRD revealed 2θ peaks at 21.25°, 21.56°, 23.14°, and for ZnO at 31.74°, 33.24°, 34.29°, 36.23°. The FT-IR demonstrated the vibrational modes of functional groups: -OH stretching, symmetric and antisymmetric -CH stretching, C = C stretching, and C-O stretching. The elemental composition of samples has been analyzed using Energy Dispersive x-ray spectroscop (EDX), and the high percentage of zinc in the composite shows a good loading rate of ZnO on the rGO's surface. By morphological investigation, monolayer sheet structures of rGO loaded with clusters of ZnO are clearly demonstrated. Positive results from therapeutic assays and biocompatibility were found with reduced hemolysis and good anticoagulation abilities proved with statistical approach. Our research is distinctive because a realistic formulation of an rGO-ZnO skin care cream with enhanced therapeutic properties, such as effective stability, spreadability, and significant moisture retention, can be recommended.
金属基纳米粒子因其独特的特性而受到广泛研究。其中，氧化锌纳米粒子在生物医学领域有众多应用。龙船花的植物提取物被用于合成氧化锌纳米粒子，取代了有害还原化学物质的使用。在当前研究中，粟的生物废料中的含碳物质通过改进的Hummer法用于合成氧化石墨烯（GO）。合成的GO通过使用牧豆树的植物提取物的绿色纳米技术转化为还原氧化石墨烯。还原氧化石墨烯-氧化锌纳米复合材料（rGO）-ZnO纳米复合材料的合成涉及一种简单、经济的一步磁搅拌法。紫外可见光谱用于表征合成材料，氧化锌（ZnO）的最大吸光度范围为384nm，rGO-ZnO复合材料的最大吸光度范围分别为243nm和366nm。X射线衍射（XRD）显示ZnO在31.54°、34.22°和36.08°处有2θ峰。对于rGO-ZnO复合材料中的还原氧化石墨烯（rGO），XRD显示在21.25°、21.56°、23.14°处有2θ峰，对于ZnO在31.74°、33.24°、34.29°、36.23°处有2θ峰。傅里叶变换红外光谱（FT-IR）展示了官能团的振动模式：-OH伸缩振动、对称和不对称-CH伸缩振动、C = C伸缩振动和C-O伸缩振动。使用能量色散X射线光谱仪（EDX）分析了样品的元素组成，复合材料中高比例的锌表明ZnO在rGO表面的良好负载率。通过形态学研究，清楚地展示了负载有ZnO簇的rGO的单层片状结构。治疗试验和生物相容性的阳性结果表明溶血减少，通过统计学方法证明具有良好的抗凝能力。我们的研究具有独特性，因为可以推荐一种具有增强治疗特性（如有效稳定性、铺展性和显著保湿性）的rGO-ZnO护肤霜的实际配方。
