Rzepczyk C M, Bishop C J
Parasite Immunol. 1984 Sep;6(5):443-57. doi: 10.1111/j.1365-3024.1984.tb00815.x.
Neutrophils in the presence of serum from dogs with occult Dirofilaria immitis infections were shown to be cytotoxic to D. immitis microfilariae recovered from the blood of microfilaraemic dogs. This cytotoxicity was correlated with the presence of IgM and IgG antibodies on the cuticular surface of microfilariae incubated in the sera from occult dogs. Such antibodies were not observed on the surface of microfilariae incubated in sera from microfilaraemic or normal dogs. The neutrophil attack was directed at the cuticular crypts, at which sites the worms appeared to be structurally most vulnerable because of the absence of the outer layer of the cuticle. The IgM antibodies were shown to be bound preferentially to these sites. Our data suggested that the neutrophil-mediated toxicity involved both hydrogen peroxide release and degranulation.
研究表明，在患有隐匿性犬恶丝虫感染的犬血清存在的情况下，中性粒细胞对从微丝蚴血症犬血液中回收的犬恶丝虫微丝蚴具有细胞毒性。这种细胞毒性与在隐匿性感染犬血清中孵育的微丝蚴角质层表面存在IgM和IgG抗体相关。在微丝蚴血症犬或正常犬血清中孵育的微丝蚴表面未观察到此类抗体。中性粒细胞的攻击针对角质层隐窝，由于角质层外层缺失，虫体在这些部位似乎在结构上最易受损。已证明IgM抗体优先结合于这些部位。我们的数据表明，中性粒细胞介导的毒性涉及过氧化氢释放和脱颗粒。
