Hilakivi L A, Hilakivi I T
Behav Brain Res. 1986 Mar;19(3):241-8. doi: 10.1016/0166-4328(86)90024-0.
The aim of this study was to determine criteria for classification of sleep-wake behavior in newborn rats by recording total body and respiratory movements with a static charge sensitive bed (SCSB). Initially, the sleep-wake behavior was simultaneously observed visually and recorded by the SCSB mattress, from the fourth to the twenty-second postnatal days. Thus, the criteria for scoring the SCSB recordings were obtained by comparison of the behavioral scorings with the SCSB tracings. To further validate this movement sensitive method for neonatal sleep studies in rats, deep neck muscle electrograms were studied at the age range of 8-13 days, and electroencephalograms as well as electromyograms from the age of 12-17 days, simultaneously with the SCSB recordings. Finally, the sleep-wake behavior of other rat pups was recorded only with the SCSB from the fourth to the twenty-second postnatal days. These latter recordings were afterwards scored by using the criteria defined in the first part of this study. The results thus obtained indicate that the SCSB method is sensitive and reliable in recording and classifying the sleep-wake behavior of newborn rats.
本研究的目的是通过使用静态电荷敏感床（SCSB）记录全身和呼吸运动，确定新生大鼠睡眠-觉醒行为的分类标准。最初，从出生后第4天到第22天，通过视觉同时观察睡眠-觉醒行为，并由SCSB床垫进行记录。因此，通过将行为评分与SCSB记录进行比较，获得了SCSB记录的评分标准。为了进一步验证这种对大鼠新生儿睡眠研究敏感的运动方法，在8-13天龄时研究了深部颈部肌肉电图，并在12-17天龄时同时研究了脑电图和肌电图，同时进行SCSB记录。最后，仅在出生后第4天到第22天使用SCSB记录其他幼鼠的睡眠-觉醒行为。随后，使用本研究第一部分中定义的标准对这些记录进行评分。由此获得的结果表明，SCSB方法在记录和分类新生大鼠的睡眠-觉醒行为方面是敏感且可靠的。
