Minuk G Y, Angus M, Brickman C M, Lawley T J, Frank M M, Hoofnagle J H, Jones E A
Gastroenterology. 1985 Jan;88(1 Pt 1):166-70. doi: 10.1016/s0016-5085(85)80149-9.
The ability of fixed macrophages of the reticuloendothelial system to clear circulating immune complexes was studied in 6 patients with primary sclerosing cholangitis, 5 patients with various other forms of chronic liver disease, and 12 normal control subjects. Autologous red cells were radiolabeled with 51Cr and sensitized with anti-Rh(D) immunoglobulin G in vitro. After intravenous infusion of the labeled antibody-coated red cells, the radioactivity content of timed blood specimens was measured. The time required by the reticuloendothelial system to clear one-half the labeled cells from the circulation (t1/2) was then determined. The t1/2 clearance times were significantly prolonged in all 6 patients with primary sclerosing cholangitis, whereas the clearance times in 4 of the 5 liver disease control patients were either normal or shortened. Serum immunoglobulin G and immunoglobulin M immune complex levels did not correlate with t1/2 clearance times. These results suggest that in primary sclerosing cholangitis there is a defect in the ability of fixed macrophages of the reticuloendothelial system to mediate clearance of circulating particles that have been opsonized with immunoglobulin G. This finding further supports recent data that incriminates the immune system in the pathogenesis of primary sclerosing cholangitis.
对6例原发性硬化性胆管炎患者、5例其他各种形式慢性肝病患者及12名正常对照者，研究了网状内皮系统中固定巨噬细胞清除循环免疫复合物的能力。体外将自体红细胞用51Cr进行放射性标记，并用抗Rh(D)免疫球蛋白G致敏。静脉输注标记的抗体包被红细胞后，测定定时采集血标本的放射性含量。然后确定网状内皮系统从循环中清除一半标记细胞所需的时间（t1/2）。所有6例原发性硬化性胆管炎患者的t1/2清除时间均显著延长，而5例肝病对照患者中有4例的清除时间正常或缩短。血清免疫球蛋白G和免疫球蛋白M免疫复合物水平与t1/2清除时间无关。这些结果表明，在原发性硬化性胆管炎中，网状内皮系统的固定巨噬细胞介导清除已被免疫球蛋白G调理的循环颗粒的能力存在缺陷。这一发现进一步支持了最近将免疫系统归咎于原发性硬化性胆管炎发病机制的相关数据。
