Li Pei-Jun, Xie Run-Sheng, Pan Jiang-Juan, Jiang Yu-Qiu, Liu Xing
Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, College of Food Science & Technology, Shaoguan University Shaoguan 512005 China.
College of Chemistry and Bioengineering, Guilin University of Technology Guilin 541004 China.
RSC Adv. 2024 Dec 6;14(52):38582-38589. doi: 10.1039/d4ra07060g. eCollection 2024 Dec 3.
Pectin-based silver nanoparticles (AgNPs) have been used in the field of antibacterials for food due to their excellent antibacterial properties. Herein, in order to achieve higher antibacterial performance, AgNPs were synthesized using high-methoxyl pectin (HMP) and amidated low-methoxyl pectin (ALMP) as precursors. Initially, ALMP-1, -2, and -4 were obtained by pectin amidation with increasing concentrations of NHOH. Later, HMP and ALMPs were used to prepare AgNPs, and their physicochemical property and antibacterial activities were studied. Transmission electron microscopy (TEM) showed that the mean diameters of HMP-Ag and ALMP-4-Ag were 11.9 ± 3.8 and 13.0 ± 5.4 nm, respectively. EDS analysis revealed that ALMP-4-Ag combined with more Ag element than HMP-Ag. X-ray photoelectron spectroscopy (XPS) indicated that ALMP-4-Ag led to a lower ratio of Ag to Ag on the surface of AgNPs. Interestingly, ALMP-4-Ag had the strongest antimicrobial effect against and , with the lowest inhibitory concentrations (MICs) of up to 33 μg mL, which was 16-fold enhanced compared with HMP-Ag (MICs = 533 μg mL). Finally, ALMP-4-Ag-treated cells revealed higher levels of protein and sugar leakage as well as increased levels of reactive oxygen species (ROS) and malondialdehyde (MDA) than HMP-Ag.
基于果胶的银纳米颗粒（AgNPs）因其优异的抗菌性能已被应用于食品抗菌领域。在此，为了获得更高的抗菌性能，以高甲氧基果胶（HMP）和酰胺化低甲氧基果胶（ALMP）为前驱体合成了AgNPs。首先，通过用浓度递增的NHOH对果胶进行酰胺化得到了ALMP-1、-2和-4。随后，用HMP和ALMPs制备AgNPs，并研究了它们的物理化学性质和抗菌活性。透射电子显微镜（TEM）显示，HMP-Ag和ALMP-4-Ag的平均直径分别为11.9±3.8和13.0±5.4nm。能谱分析（EDS）表明，ALMP-4-Ag比HMP-Ag结合了更多的银元素。X射线光电子能谱（XPS）表明，ALMP-4-Ag导致AgNPs表面的Ag⁺/Ag比例更低。有趣的是，ALMP-4-Ag对[具体菌种1]和[具体菌种2]具有最强的抗菌作用，最低抑菌浓度（MICs）高达33μg/mL，与HMP-Ag（MICs = 533μg/mL）相比提高了16倍。最后，与HMP-Ag相比，经ALMP-4-Ag处理的细胞显示出更高水平的蛋白质和糖泄漏以及活性氧（ROS）和丙二醛（MDA）水平的增加。
