Pașca Sergiu P, Arlotta Paola, Bateup Helen S, Camp J Gray, Cappello Silvia, Gage Fred H, Knoblich Jürgen A, Kriegstein Arnold R, Lancaster Madeline A, Ming Guo-Li, Novarino Gaia, Okano Hideyuki, Parmar Malin, Park In-Hyun, Reiner Orly, Song Hongjun, Studer Lorenz, Takahashi Jun, Temple Sally, Testa Giuseppe, Treutlein Barbara, Vaccarino Flora M, Vanderhaeghen Pierre, Young-Pearse Tracy
Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.
Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA.
Nature. 2025 Mar;639(8054):315-320. doi: 10.1038/s41586-024-08487-6. Epub 2024 Dec 9.
As the field of neural organoids and assembloids expands, there is an emergent need for guidance and advice on designing, conducting and reporting experiments to increase the reproducibility and utility of these models. In this Perspective, we present a framework for the experimental process that encompasses ensuring the quality and integrity of human pluripotent stem cells, characterizing and manipulating neural cells in vitro, transplantation techniques and considerations for modelling human development, evolution and disease. As with all scientific endeavours, we advocate for rigorous experimental designs tailored to explicit scientific questions as well as transparent methodologies and data sharing to provide useful knowledge for current research practices and for developing regulatory standards.
随着神经类器官和类组装体领域的不断扩展，迫切需要在设计、开展和报告实验方面获得指导与建议，以提高这些模型的可重复性和实用性。在本观点文章中，我们提出了一个实验流程框架，该框架涵盖确保人类多能干细胞的质量和完整性、在体外对神经细胞进行表征和操作、移植技术以及模拟人类发育、进化和疾病的注意事项。与所有科学研究一样，我们提倡针对明确的科学问题进行严谨的实验设计，以及采用透明的方法和数据共享，以便为当前的研究实践和制定监管标准提供有用的知识。
