Ben Tekaya Seifeddine, Nouioui Imen, Flores Gabryelle May, Neumann-Schaal Meina, Bredoire Felix, Basile Franco, van Diepen Linda T A, Ward Naomi L
Department of Molecular Biology, University of Wyoming, 1000 E. University Avenue, Laramie, WY 82071, USA.
Leibniz Institute, DSMZ-German Collection of Microorganisms and Cell Cultures, Berlin, Germany.
Int J Syst Evol Microbiol. 2024 Dec;74(12). doi: 10.1099/ijsem.0.006603.
A novel Gram-stain-positive, black-pigmented bacterium, designated as WL48A , was isolated from the surface of badland sedimentary rock in the Red Desert of Wyoming and characterized using a polyphasic taxonomic approach. Good growth occurred at 28-32 °C, pH 7-9, and NaCl less than 1% (w/v). Colonies, growing well on International Streptomyces Project media (ISP) 3 and ISP 7, were black and adhering to the agar. Phylogenetic analyses based on 16S rRNA gene and draft genome sequences showed that strain WL48A belongs to the family , forming a distinct sub-branch with DSM 46841. The organism showed 16S rRNA gene sequence similarity of 98.8% with DSM 46841. Digital DNA-DNA hybridization value between the genome sequences of strain WL48A and DSM 46841 was 51.8%, below the threshold of 70% for prokaryotic species delineation. The chemotaxonomic investigation revealed the presence of galactose, glucose, mannose, xylose and ribose as well as -DAP in the peptidoglycan layer. The polar lipid profiles contained phosphatidylcholine (PC), phosphatidylinositol (PI), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) phosphoglycolipid, phospholipids and an unidentified lipid. The menaquinone profile consisted of MK-9(H) (98.2%) and MK-9(H) (10.8%). The major fatty acid profile (>15%) comprised iso-C and iso-C. Based on phenotypic, genetic and genomic data, strain WL48A (=DSM 116197 = NCIMB 15483=NCCB 100957 =ATCC TSD-376) merits to be considered as a novel species for which the name sp. nov. is proposed.
从怀俄明州红沙漠的荒地沉积岩表面分离出一株新型革兰氏阳性、黑色色素细菌，命名为WL48A，并采用多相分类方法对其进行了表征。该菌在28-32°C、pH 7-9和NaCl含量低于1%（w/v）的条件下生长良好。在国际链霉菌项目培养基（ISP）3和ISP 7上生长良好的菌落为黑色，且附着于琼脂上。基于16S rRNA基因和基因组草图序列的系统发育分析表明，菌株WL48A属于该科，与DSM 46841形成一个独特的亚分支。该菌与DSM 46841的16S rRNA基因序列相似性为98.8%。菌株WL48A与DSM 46841基因组序列之间的数字DNA-DNA杂交值为51.8%，低于原核生物物种界定的70%阈值。化学分类学研究表明，肽聚糖层中存在半乳糖、葡萄糖、甘露糖、木糖和核糖以及二氨基庚二酸。极性脂质谱包含磷脂酰胆碱（PC）、磷脂酰肌醇（PI）、二磷脂酰甘油（DPG）、磷脂酰乙醇胺（PE）、磷酸糖脂、磷脂和一种未知脂质。甲基萘醌谱由MK-9（H）（98.2%）和MK-9（H）（10.8%）组成。主要脂肪酸谱（>15%）包括异-C和异-C。基于表型、遗传和基因组数据，菌株WL48A（=DSM 116197 = NCIMB 15483=NCCB 100957 =ATCC TSD-376）应被视为一个新物种，为此提出新名称sp. nov.。
