Yuan Cansheng, Ruan Luyao, Yang Ying, Wang Lin, Ke Zhuang, He Jian
College of Rural Revitalization, Jiangsu Open University, Nanjing, 210095, Jiangsu, People's Republic of China.
College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, People's Republic of China.
Curr Microbiol. 2025 Sep 20;82(11):514. doi: 10.1007/s00284-025-04502-9.
A Gram-stain-positive bacterium, which produced indole-3-acetic acid (IAA) and inhibited Rhizoctornia solani Kühn, designated strain P4, was isolated from agricultural soil in Wuxi city, Jiangsu province, PR China. Cells were rod-shaped, facultatively anaerobic, endospore-forming, motile by peritrichous flagella. Growth occurred at 15-42 °C (optimum at 30 °C), pH 5.0-9.0 (optimum pH 7.0-7.5) and with 0-3.0% NaCl (w/v; optimum, 0.5%). The 16S rRNA gene sequence analysis showed that strain P4 is a member of the genus Paenibacillus, closely related to Paenibacillus alvei DSM 29 (98.3%), followed by P. marinisediminis S27 (96.0%), P. aquistagni 11 (95.9%) and P. profundus SI 79 (95.5%). Phylogenetic trees based on 16S rRNA gene and genomic sequences indicated that strain P4 was clustered in the genus Paenibacillus and formed a subclade with P. alvei DSM 29. The genome size of P4 was 6.7 Mb with a G + C content of 46.6%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the genomes of strain P4 and seven closely related type strains ranged from 71.5-84.8% to 20.6-28.7%, respectively. The percentage of conserved proteins (POCP) values between strain P4 and its related strains ranged from 57.2 to 73.5%. The major fatty acids (≥ 5%) were anteiso-C, iso-C, C, iso-C and anteiso-C. The polar lipids profile consisted of phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), phosphatidylglycerol (PG), unidentified aminolipid (AL), three unidentified glycolipids (GL1-3), three unidentified phospholipids (PL1-3), five unidentified aminophospholipids (APL1-5) and five unidentified lipids (L1-5). The only isoprenoid quinone was menaquinone-7 (MK-7) and the cell wall peptidoglycan was meso-DAP. Based on phylogenetic, genomic and phenotypic data, strain P4 was considered to represent a novel species in genus Paenibacillus, for which the name Paenibacillus wuxiensis was proposed, with P4 (= MCCC 1K09189 = KCTC 43706) as the type strain.
从中国江苏省无锡市的农业土壤中分离出一株革兰氏阳性菌，该菌能产生吲哚 - 3 - 乙酸（IAA）并抑制立枯丝核菌，命名为菌株P4。细胞呈杆状，兼性厌氧，产芽孢，周身鞭毛运动。在15 - 42℃（最适温度30℃）、pH 5.0 - 9.0（最适pH 7.0 - 7.5）和0 - 3.0% NaCl（w/v；最适0.5%）条件下生长。16S rRNA基因序列分析表明，菌株P4是芽孢杆菌属的成员，与蜂房芽孢杆菌DSM 29密切相关（98.3%），其次是海泥芽孢杆菌S27（96.0%）、水城芽孢杆菌11（95.9%）和深海芽孢杆菌SI 79（95.5%）。基于16S rRNA基因和基因组序列构建的系统发育树表明，菌株P4聚类在芽孢杆菌属中，并与蜂房芽孢杆菌DSM 29形成一个亚分支。P4的基因组大小为6.7 Mb，G + C含量为46.6%。菌株P4与七个密切相关模式菌株的基因组之间的平均核苷酸同一性（ANI）和数字DNA - DNA杂交（dDDH）值分别在71.5 - 84.8%和20.6 - 28.7%之间。菌株P4与其相关菌株之间的保守蛋白百分比（POCP）值在57.2%至73.5%之间。主要脂肪酸（≥5%）为anteiso - C、iso - C、C、iso - C和anteiso - C。极性脂质谱由磷脂酰乙醇胺（PE）、磷脂酰单甲基乙醇胺（PME）、磷脂酰甘油（PG）、未鉴定的氨基脂质（AL）、三种未鉴定的糖脂（GL1 - 3）、三种未鉴定的磷脂（PL1 - 3）、五种未鉴定的氨基磷脂（APL1 - 5）和五种未鉴定的脂质（L1 - 5）组成。唯一的类异戊二烯醌是甲基萘醌 - 7（MK - 7），细胞壁肽聚糖为内消旋二氨基庚二酸。基于系统发育、基因组和表型数据，菌株P4被认为代表芽孢杆菌属中的一个新物种，为此提出了无锡芽孢杆菌这一名称，以P4（= MCCC 1K09189 = KCTC 43706）作为模式菌株。
