Pitcock J A, Brown P S, Brooks B, Rapp J P, Rightsel W, Muirhead E E
Exp Mol Pathol. 1985 Feb;42(1):29-43. doi: 10.1016/0014-4800(85)90016-4.
The renomedullary interstitial cell (RIC) has been implicated in the antihypertensive action of the kidney. This cell has been isolated in tissue culture and shown to have an antihypertensive action in several models of experimental hypertension. Morphometric studies of RIC in vivo from Dahl rats sensitive and resistant to the hypertensive effects of high-salt diets indicate major differences between the RICs. These cells were therefore isolated from salt-sensitive and salt-resistant strains of rat, grown, and maintained in tissue culture. Major morphologic differences between the two cell lines were noted and persisted for multiple tissue culture passages. The cells from resistant animals were larger and had more lipid granules. These differences were similar to those seen in vivo. In short-term experiments these cells were compared for their antihypertensive effect. The two cell lines were injected subcutaneously into two groups of hypertensive recipient rats, one group of Dahl salt-sensitive rats on a high-salt diet and one group of Wistar rats subjected to the one-kidney, one-clip Goldblatt procedure. In both cases differences were noted between the cell lines. These data support the concept that differences between the Dahl salt-sensitive and salt-resistant rats may be related to variations in their RIC.
肾髓质间质细胞（RIC）与肾脏的降压作用有关。这种细胞已在组织培养中分离出来，并在几种实验性高血压模型中显示出具有降压作用。对高盐饮食高血压效应敏感和耐受的Dahl大鼠体内的RIC进行形态计量学研究表明，RIC之间存在主要差异。因此，从大鼠的盐敏感和盐抵抗品系中分离出这些细胞，在组织培养中生长并维持。注意到两种细胞系之间存在主要的形态学差异，并且在多次组织培养传代中持续存在。来自抗性动物的细胞更大，并且有更多的脂质颗粒。这些差异与在体内观察到的差异相似。在短期实验中，比较了这些细胞的降压效果。将这两种细胞系皮下注射到两组高血压受体大鼠中，一组是高盐饮食的Dahl盐敏感大鼠，另一组是接受单肾单夹Goldblatt手术的Wistar大鼠。在这两种情况下，细胞系之间都存在差异。这些数据支持这样一种概念，即Dahl盐敏感大鼠和盐抵抗大鼠之间的差异可能与其RIC的变化有关。
