Pitcock J A, Brown P S, Byers W, Brooks B, Muirhead E E
Hypertension. 1981 Nov-Dec;3(6 Pt 2):II-75-80. doi: 10.1161/01.hyp.3.6_pt_2.ii-75.
It was demonstrated earlier that the renal venous effluent of one-kidney, one clip hypertensive rats contained a vasodepressor lipid resembling the antihypertensive neutral renomedullary lipid (ANRL), following unclipping and as the arterial pressure (MAP) was lowered. Consequently, the sham-unclipped (clip-intact) and the unclipped kidney (CK and UCK) were studied by electronmicroscopy and morphometrically (Weibel's techniques). Renomedullary interstitial cells (RIC) of the CK had abundant granules. The collecting duct (CD) had tall lining cells containing pale granules and displayed intercellular channels. Following unclipping, the RIC degranulated and the CD cells became flattened, lost their pale granules, and the intercellular channels disappeared as the MAP decreased. These changes were evident by EM appearance and volume density measurements. The renopapillary changes occurred as the kidney secreted the ANRL-like substance into the blood.
先前的研究表明，在解除单侧肾动脉夹闭且动脉压（平均动脉压，MAP）降低后，单侧肾动脉夹闭高血压大鼠的肾静脉流出液中含有一种血管舒张降压脂质，类似于抗高血压的中性肾髓质脂质（ANRL）。因此，对假手术未夹闭（夹闭完整）和未夹闭的肾脏（对照肾和解除夹闭肾）进行了电子显微镜检查和形态计量学研究（采用韦贝尔技术）。对照肾的肾髓质间质细胞（RIC）有丰富的颗粒。集合管（CD）有高柱状衬里细胞，含有浅色颗粒，并显示细胞间通道。解除夹闭后，随着平均动脉压降低，肾髓质间质细胞脱颗粒，集合管细胞变扁平，并失去其浅色颗粒，细胞间通道消失。这些变化通过电子显微镜观察和体积密度测量得以明确。肾乳头的变化是在肾脏将类似ANRL的物质分泌到血液中时发生的。
