Stevens Dinny, Charlton-Sevcik Amanda K, Braswell W Evan, Sayes Christie M
Department of Environmental Science, Baylor University, Waco, Texas 76798, United States.
Insect Management and Molecular Diagnostics Laboratory, USDA APHIS PPQ S&T, Edinburg, Texas 78541, United States.
ACS Appl Mater Interfaces. 2025 Jan 8;17(1):322-332. doi: 10.1021/acsami.4c15245. Epub 2024 Dec 16.
Engineered nanoparticles are precisely synthesized to exploit unique properties conferred by their small size and high surface area for environmental, biomedical, and agricultural applications. While these physical properties dictate functionality, they can also have various intended and unintended implications for biological systems. Both the particle size and shape influence cellular uptake. Because of zinc's antibacterial properties and role as a plant micronutrient, polyvinylpyrrolidone stabilized zinc nanoparticles (ZnNP) were selected for this study. Four synthesis methods were tested to produce distinct size populations of polymer-coated ZnNP, and all utilized water as the solvent to promote sustainable, green chemistry. The antibacterial activity of ZnNP was assessed in two agriculturally relevant bacteria strains: and . To further examine the effects of ZnNP on bacterial cells, reactive oxygen species (ROS) generation was measured via hydrogen peroxide (HO) production. The bacteria's incubation temperature was also altered to assess bacterial growth and susceptibility after exposure to ZnNP. The ZnNP from the smaller size population inhibited the most growth across bacterial strains, assays, and incubation temperatures. Increased antibacterial effects and ROS production were observed after incubation at a higher temperature. These results indicate that the deliberately designed nanoparticles are potentially valuable in microbial control and offer promising solutions for the future of healthy agricultural systems.
工程纳米颗粒是精确合成的，以利用其小尺寸和高表面积赋予的独特性质用于环境、生物医学和农业应用。虽然这些物理性质决定了其功能，但它们对生物系统也可能有各种预期和非预期的影响。颗粒的大小和形状都会影响细胞摄取。由于锌的抗菌特性及其作为植物微量营养素的作用，本研究选择了聚乙烯吡咯烷酮稳定的锌纳米颗粒（ZnNP）。测试了四种合成方法以制备不同尺寸分布的聚合物包覆ZnNP，并且所有方法都使用水作为溶剂以促进可持续的绿色化学。在两种与农业相关的细菌菌株中评估了ZnNP的抗菌活性： 和 。为了进一步研究ZnNP对细菌细胞的影响，通过过氧化氢（HO）产生来测量活性氧（ROS）的生成。还改变了细菌的培养温度，以评估暴露于ZnNP后的细菌生长和敏感性。较小尺寸分布的ZnNP在各种细菌菌株、检测方法和培养温度下抑制的生长最多。在较高温度下培养后，观察到抗菌效果增强和ROS产生增加。这些结果表明，经过精心设计的纳米颗粒在微生物控制方面具有潜在价值，并为健康农业系统的未来提供了有前景的解决方案。
