Alwaleed Eman A, Alzain Mashail N, Loutfy Naglaa, El-Shahir Amany A
Department of Botany and Microbiology, Faculty of Science, South Valley University, Qena 83523, Egypt.
Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11761, Saudi Arabia.
Plants (Basel). 2025 Jul 8;14(14):2104. doi: 10.3390/plants14142104.
The present study aimed to identify the active chemical compounds, mainly phenolic acids, of and , evaluate the pharmacokinetic properties of their primary compounds, and assess a novel method for the biocontrol of blue mold by evaluating the antifungal activity of both extracts. Gas chromatography (GC) and high-performance liquid chromatography (HPLC) were utilized to identify the active chemical compounds, mainly phenolic acids. GC illustrated the presence of long-chain aliphatic fatty acids like eicosanoic acid with the formation of oct-1-en-3-ol compounds attached. Catechin was the main bioactive component among the several bioactive compounds identified by HPLC analysis, exhibiting favorable pharmacokinetic behavior, including good absorption, distribution, and metabolic stability. According to the findings, both extracts had antifungal activity, but extract (100 mg/mL) exhibited the strongest in vitro and in vivo antifungal activity, with the highest percentages of inhibition (disk diffusion method) against , , and , ranging between 62.67 and 100%. extract (100 mg/mL) could fully inhibit the pathogenicity and aggressiveness of the five tested strains in apple fruits (in vivo). In conclusion, the extract from and shows potential antifungal properties and a high phytochemical content.
本研究旨在鉴定[具体植物名称1]和[具体植物名称2]的活性化学成分，主要是酚酸，评估其主要化合物的药代动力学特性，并通过评估两种提取物的抗真菌活性来评估一种新型的青霉生物防治方法。利用气相色谱（GC）和高效液相色谱（HPLC）来鉴定活性化学成分，主要是酚酸。GC显示存在长链脂肪族脂肪酸，如二十烷酸，并伴有附着的1-辛烯-3-醇化合物的形成。儿茶素是HPLC分析鉴定出的几种生物活性化合物中的主要生物活性成分，表现出良好的药代动力学行为，包括良好的吸收、分布和代谢稳定性。根据研究结果，两种提取物均具有抗真菌活性，但[具体植物名称1]提取物（100mg/mL）在体外和体内均表现出最强的抗真菌活性，对[具体真菌名称1]、[具体真菌名称2]和[具体真菌名称3]的抑制率最高（纸片扩散法），介于62.67%至100%之间。[具体植物名称1]提取物（100mg/mL）可完全抑制苹果果实中五种受试菌株的致病性和侵袭性（体内）。总之，[具体植物名称1]和[具体植物名称2]的提取物显示出潜在的抗真菌特性和高植物化学含量。
