Hekimoglu Emine Rumeysa, Esrefoglu Mukaddes, Cimen Fatma Bedia Karakaya, Pasin Özge, Dedeakayogullari Huri
Department of Histology and Embryology, Faculty of Medicine, Bezmialem Vakıf University, Fatih, Istanbul, Turkey.
Department of Biostatistics, Faculty of Medicine, Bezmialem Vakif University, Istanbul, Turkey.
Aesthetic Plast Surg. 2024 Dec 16. doi: 10.1007/s00266-024-04554-5.
Adipose tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration. It can also give rise to many multipotent adipose-derived stromal cells. SVF is the population of cells obtained from mechanical or enzymatic digestion of lipoaspirate with no necessity for cell culture or expansion. Recently, the heterogeneous cell population found in the SVF gained wide-ranging translational significance in regenerative medicine.
Forty-eight male rats were randomly divided into two main groups, including the control and SVF groups. Each group was further divided into four groups as follows: 0th-, 3rd-, 7th-, and 10th-day groups. A skin excision of 1 × 1 cm covering the epidermis and dermis was performed on the back skin. Just after the wound was created, a subepidermal injection of SVF was applied. SVF was obtained from human adipose tissue using Lipocube SVFTM. On the 0th (1 h after the injections), 3rd, 7th, and 10th days, rats were killed, and skin excisions from the wound areas tissues were performed. Histopathological, biochemical, and western blotting analyses were performed on tissues.
Our data showed that SVF obtained from a healthy woman improved wound healing in healthy rats. SVF has promoted wound healing mainly because of its antioxidant, antiapoptotic, and fibroblast/myofibroblast stimulating effects. SVF stimulated collagen production and contraction of the wound lips, supporting the closure.
Our study provides additional data about the efficacy and pathophysiological and molecular mechanisms of the action of SVF on wound healing in healthy subjects. Our study is an experimental animal study.
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脂肪组织为即时给药提供了丰富的基质血管成分（SVF）细胞来源。它还能产生许多多能脂肪来源的基质细胞。SVF是通过对脂肪抽吸物进行机械或酶消化获得的细胞群体，无需细胞培养或扩增。最近，在SVF中发现的异质性细胞群体在再生医学中具有广泛的转化意义。
48只雄性大鼠随机分为两个主要组，即对照组和SVF组。每组再进一步分为四组，如下：第0天、第3天、第7天和第10天组。在背部皮肤进行1×1厘米的皮肤切除，包括表皮和真皮。伤口形成后立即进行皮下注射SVF。使用Lipocube SVFTM从人脂肪组织中获取SVF。在第0天（注射后1小时）、第3天、第7天和第10天，处死大鼠，并对伤口区域组织进行皮肤切除。对组织进行组织病理学、生化和蛋白质印迹分析。
我们的数据表明，从健康女性获得的SVF可改善健康大鼠的伤口愈合。SVF促进伤口愈合主要是因为其抗氧化、抗凋亡以及刺激成纤维细胞/肌成纤维细胞的作用。SVF刺激胶原蛋白生成和伤口边缘收缩，促进伤口闭合。
我们的研究提供了关于SVF对健康受试者伤口愈合作用的疗效、病理生理和分子机制的更多数据。我们的研究是一项实验动物研究。
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