Li Lingyi, Zhang Shanbo, Yu Bin, Liu Shao Quan, Xiong Yancai
Department of Food Science and Technology, National University of Singapore, S14 Level 5, Science Drive 2, Singapore 117542, Singapore.
Mane SEA Pte Ltd., 3 Biopolis Drive, #07-17/18/19 Synapse, Singapore 138623, Singapore.
Foods. 2024 Nov 29;13(23):3861. doi: 10.3390/foods13233861.
Due to the structural diversity of flavonoids in functional plant foods and the inherent limitations of existing techniques, it is important to develop a simple and green (environmentally friendly) method of extracting flavonoids from plant foods. In this study, a method involving solvent extraction followed by automated solid-phase extraction was developed for extracting flavonoids from flos (JYH) and (SYH), both of which are widely used functional plant-based foods in Asian countries. For the optimisation of the solvent extraction method, solvent concentration (0.0, 20.0, 40.0, 60.0, 80.0 and 100.0% (/) of ethanol-water solution), extraction temperature (40, 60 and 80 °C) and extraction time (15.0, 30.0, 60.0, 90.0 and 120.0 min) were evaluated via design of experiment after screening. For solid-phase extraction, five cartridges (Strata-X, InertSep RP-2, InertSep RP-C18, Bond Elut-ENV, Oasis Prime HLB) were evaluated and different elution steps were optimised to obtain high recoveries (79.69-140.67%) for eight target flavonoids, including rutin, isoquercetin and luteolin. Antioxidant capacity assays revealed that JYH samples demonstrated superior antioxidant potential compared to SYH. The optimised extraction method provides a valuable tool for industrial-scale flavonoid production.
由于功能性植物性食品中黄酮类化合物的结构多样性以及现有技术的固有局限性，开发一种从植物性食品中提取黄酮类化合物的简单且绿色（环境友好）的方法非常重要。在本研究中，开发了一种先进行溶剂萃取然后进行自动固相萃取的方法，用于从菊花（JYH）和桑叶（SYH）中提取黄酮类化合物，这两种都是亚洲国家广泛使用的功能性植物性食品。为了优化溶剂萃取方法，在筛选后通过实验设计评估了溶剂浓度（乙醇 - 水溶液的0.0、20.0、40.0、60.0、80.0和100.0%（v/v））、萃取温度（40、60和80°C）以及萃取时间（15.0、30.0、60.0、90.0和120.0分钟）。对于固相萃取，评估了五个柱（Strata - X、InertSep RP - 2、InertSep RP - C18、Bond Elut - ENV、Oasis Prime HLB），并优化了不同的洗脱步骤，以获得包括芦丁、异槲皮素和木犀草素在内的八种目标黄酮类化合物的高回收率（79.69 - 140.67%）。抗氧化能力测定表明，JYH样品的抗氧化潜力优于SYH。优化后的提取方法为工业规模的黄酮类化合物生产提供了一个有价值的工具。
