Liu Dong-Huan, Ji Wen-Tao, Zou Qing-Qing, Wu Han-Yu, Li Tao, Shi Wen-Bin, Jiang Chuang-Dao
Beijing Botanical Garden, Key Laboratory of National Forestry and Grassland Administration on Plant Ex Situ Conservation, Beijing 100093, China.
State Key Laboratory of Plant Diversity and Specialty Crops, China National Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Int J Mol Sci. 2024 Nov 29;25(23):12876. doi: 10.3390/ijms252312876.
To reveal the global regulation of photosynthetic electron transport (PET) in shade plants, the changes in chlorophyll fluorescence induction kinetics (CFI) curves and proteomics were investigated using varieties. There was a significant difference in CFI curves between 'Fire Island' and other varieties (such as 'Sum and Substance') grown under weak light. Weak light induced the appearance of the W phase of CFI curves in the two varieties, which was consistent with a clear decrease in the oxygen-evolving complex and a large upregulation of photosystem (PS) II proteins. In 'Fire Island', the O-J rise of the CFI curves increased faster under weak light than under appropriate light, and this was not only accompanied by a large upregulation of the PS II protein but also a great downregulation in cytochrome b/f, plastocyanin, and PS I. Moreover, weak light resulted in a considerable increase in photosynthetic rate and Rubisco abundance in 'Fire Island', yet the non-photochemical quenching and abundance of CP24 declined significantly. By contrast, weak light had fewer effects on these parameters in 'Sum and Substance'. Therefore, we suggest that the PET is mainly affected by the abundance of PS II, oxygen-evolving complex, cytochrome b/f, plastocyanin, and PS I in plants grown under weak light; meanwhile, the improved photosynthetic capacity under weak light was mainly related to the enhancement of light energy absorption and capture of PS II as well as the increase in the abundance of Rubisco.
为揭示阴生植物光合电子传递（PET）的全局调控机制，利用多个品种研究了叶绿素荧光诱导动力学（CFI）曲线变化和蛋白质组学。在弱光下生长的‘火岛’与其他品种（如‘总和与实质’）之间，CFI曲线存在显著差异。弱光诱导这两个品种CFI曲线出现W相，这与放氧复合体的明显减少以及光系统（PS）II蛋白的大量上调一致。在‘火岛’中，弱光下CFI曲线的O-J上升比适宜光照下更快，这不仅伴随着PS II蛋白的大量上调，还伴随着细胞色素b/f、质体蓝素和PS I的大幅下调。此外，弱光导致‘火岛’光合速率和Rubisco丰度显著增加，但非光化学猝灭和CP24丰度显著下降。相比之下，弱光对‘总和与实质’这些参数的影响较小。因此，我们认为在弱光下生长的植物中，PET主要受PS II、放氧复合体、细胞色素b/f、质体蓝素和PS I丰度的影响；同时，弱光下光合能力的提高主要与PS II光能吸收和捕获的增强以及Rubisco丰度的增加有关。
