Moro Angela M, Brucker Natália, Goethel Gabriela, Flesch Ingrid, Nascimento Sabrina, Charão Mariele, Gauer Bruna, Sauer Elisa, Cestonaro Larissa V, Viçozzi Gabriel Pedroso, Gioda Adriana, Saint'Pierre Tatiana D, Arbo Marcelo D, Garcia Ingrid, Cattani Shanda A, Petrecelli Rodrigo R, Martins Mirkos Ortiz, Garcia Solange Cristina
Laboratory of Toxicology (LATOX), Department of Analysis, Faculty of Pharmacy, Federal University of Rio Grande do Sul, Rua São Luis 150-Anexo II, Santa Cecília, Porto Alegre, RS, CEP: 90610-000, Brazil.
Department of Physiology and Pharmacology, Federal University of Santa Maria, Santa Maria, RS, Brazil.
Biol Trace Elem Res. 2024 Dec 18. doi: 10.1007/s12011-024-04472-2.
Occupational exposure to pollutants may cause health-damaging effects in humans. Genotoxicity assays can be used to detect the toxic effects of pollutants. In the present study, we evaluated genetic damage in three populations occupationally exposed to benzene, pyrenes, and agrochemicals and assessed the possible influence of titanium (Ti) co-exposure. A total of 275 subjects were enrolled in this study. The occupationally exposed population was composed of 201 male individuals, divided into three different groups: gas station attendants (GSA group) (n = 76), taxi drivers (TD group) (n = 97), farmers (farmers group) (n = 28), and control (n = 74). Biomarkers of exposure and effect were investigated such as AChe, BuChE, t,t-muconic acid (t,t-MA), and 1-hydroxypyrene (1-OHP). Ti levels in blood were higher in all the workers compared with the control group. DNA damage evaluated by comet assay was higher in the taxi drivers and farmers than in the controls, and the frequency of micronucleate buccal cells was higher in the gas station attendants and taxi drivers than in the controls. Correlations were found among occupational exposure time and biomarkers of exposure, genotoxicity biomarkers, and blood Ti levels. Our results demonstrated Ti co-exposure in the gas station attendants, taxi drivers, and farmers, and blood Ti levels were linked with the respective biomarkers of exposure. Additionally, tools through machine learning corroborated these findings, and Ti was the factor that contributed to DNA damage. Thus, the present study indicates the role of Ti in occupational settings and interactions with already known major xenobiotics present in the occupational environment contributing to genotoxicity.
职业接触污染物可能会对人类健康造成损害。遗传毒性检测可用于检测污染物的毒性作用。在本研究中，我们评估了三个职业接触苯、芘和农用化学品的人群的遗传损伤，并评估了钛（Ti）共同暴露的可能影响。本研究共纳入275名受试者。职业暴露人群由201名男性个体组成，分为三个不同组：加油站工作人员（GSA组）（n = 76）、出租车司机（TD组）（n = 97）、农民（农民组）（n = 28）和对照组（n = 74）。研究了暴露和效应的生物标志物，如乙酰胆碱酯酶（AChe）、丁酰胆碱酯酶（BuChE）、反式，反式-粘康酸（t,t-MA）和1-羟基芘（1-OHP）。与对照组相比，所有工人血液中的钛水平都更高。通过彗星试验评估的DNA损伤在出租车司机和农民中高于对照组，而微核颊细胞的频率在加油站工作人员和出租车司机中高于对照组。在职业暴露时间与暴露生物标志物、遗传毒性生物标志物和血液钛水平之间发现了相关性。我们的结果表明，加油站工作人员、出租车司机和农民存在钛共同暴露，血液钛水平与各自的暴露生物标志物相关。此外，机器学习工具证实了这些发现，并且钛是导致DNA损伤的因素。因此，本研究表明了钛在职业环境中的作用以及与职业环境中已知的主要外源性物质的相互作用，这些外源性物质导致了遗传毒性。
