Chen Duo, Chen Xupeng, Zheng Xuehai, Zhu Jinmao, Xue Ting
The Public Service Platform for Industrialization Development Technology of Marine Biological Medicine and Products of the State Oceanic Administration, Fujian Key Laboratory of Special Marine Bioresource Sustainable Utilization, Southern Institute of Oceanography, Key Laboratory of Developmental and Neural Biology College of Life Sciences, Fujian Normal University, Fuzhou, China.
BMC Genomics. 2024 Dec 18;25(1):1197. doi: 10.1186/s12864-024-11125-0.
Cyclocarya paliurus is a high-value tree, and it contains a variety of bioactive secondary metabolites which have broad application prospects in medicine, food and health care. Triterpenoids can improve the bioactive function of C. paliurus health tea and also improve the efficacy of health care tea.
The results of this study showed that there were 69 kinds were terpenoids, and triterpenoids accounted for more than 80%. We excavated 5 kinds of triterpenoid metabolites with high content and significant difference dynamics, namely, corosolic acid, asiatic acid, maslinic acid, ursolic acid and oleanolic acid. The co-expression analysis identified CYP71D8 and CYP716A15 co-expressed with β-AS may generate oleanane type triterpenoids by modifying β-amyrin, while CYP71AN24 and CYP98A2 co-expressed with LUS may play a key role in lupine type triterpenoids biosynthesis. MYB,Whirly,WRKY and bHLH families, which showed strong correlation with function genes, may play an important role in the regulation of P450 and OSC expression. A total of 20 modules were identified by WGCNA analysis, and CYP71AU50 and CYP716A15 in tan and orange modules may play a major role in the synthesis of oleanolic acid, ursolic acid and asiatic acid, while CYP82D47 in lightcyan 1 module may be the hub gene for the biosynthesis of corosolic acid and maslinic acid.
Our findings mined candidate genes closely related to triterpenoid synthesis in C. paliurus. The results of this paper can provide scientific reference for breeding high-content triterpenoid varieties of C. paliurus.
青钱柳是一种具有高价值的树木，它含有多种生物活性次生代谢产物，在医药、食品和保健领域具有广阔的应用前景。三萜类化合物可以提高青钱柳保健茶的生物活性功能，也能提高保健茶的功效。
本研究结果表明，共鉴定出69种萜类化合物，其中三萜类化合物占比超过80%。我们挖掘出5种含量高且动态差异显著的三萜类代谢产物，即科罗索酸、积雪草苷、山楂酸、熊果酸和齐墩果酸。共表达分析表明，与β-AS共表达的CYP71D8和CYP716A15可能通过修饰β-香树脂醇生成齐墩果烷型三萜类化合物，而与LUS共表达的CYP71AN24和CYP98A2可能在羽扇豆烷型三萜类化合物生物合成中起关键作用。与功能基因显示出强相关性的MYB、Whirly、WRKY和bHLH家族可能在P450和OSC表达调控中发挥重要作用。通过WGCNA分析共鉴定出20个模块，棕褐色和橙色模块中的CYP71AU50和CYP716A15可能在齐墩果酸、熊果酸和积雪草苷的合成中起主要作用，而浅青色1模块中的CYP82D47可能是科罗索酸和山楂酸生物合成的枢纽基因。
我们的研究结果挖掘出了与青钱柳三萜类化合物合成密切相关的候选基因。本文结果可为青钱柳高含量三萜类化合物品种选育提供科学参考。
