Peng Zixin, Hu Yue, Ye Zehong, Deng Jiang, Yang Dajin, Xu Jin, Fanning Séamus, Liu Guihua, Li Fengqin
NHC Key Laboratory of Food Safety Risk Assessment, Chinese Academy of Medical Science Research Unit (2019RU014), China National Center for Food Safety Risk Assessment, Beijing, China.
Department of Genetics and Genome Biology, University of Leicester, Leicester, United Kingdom.
China CDC Wkly. 2024 Nov 22;6(47):1223-1231. doi: 10.46234/ccdcw2024.246.
Enterococci are considered opportunistic pathogens. However, they can serve as a reservoir of antibacterial resistance (ABR) traits and transfer these to humans through the food chain.
Antibiotic susceptibility testing and multilocus sequence typing were used to characterize the ABR and population structure of 488 enterococcal isolates recovered along the food chain from four provincial-level administrative divisions (PLADs) in China.
() was the dominant species cultured from pig farms and retail meat, while () and were dominant in patients with diarrhea and retail fruits and vegetables, respectively. Approximately 67% of all isolates were multidrug-resistant (MDR). resistance to ampicillin and penicillin was significantly higher than that of . The isolates exhibited substantially heterologous sequence types (STs), whereas isolates were clearly divided into clonal complex (CC) CC17 and CC94 clades. isolates were mainly detected in hospitalized children and were identified as the hospital-associated CC17 clade with ampicillin and penicillin resistance. Notably, ST16 and ST65 and ST60 and ST94 detected in patients with diarrhea were also detected in farm and food samples, indicating that these STs should be closely monitored. The community-lineage CC94 clade was detected in patients with diarrhea, implying that community isolates might find their way into hospitals.
This study highlights the One Health challenges posed by enterococci important to human health and the need to implement integrated preventive measures for their control.
肠球菌被认为是机会性病原体。然而，它们可作为抗菌耐药性（ABR）特征的储存库，并通过食物链将这些特征传递给人类。
采用抗生素敏感性试验和多位点序列分型来表征从中国四个省级行政区（PLADs）的食物链中分离出的488株肠球菌的ABR和种群结构。
（）是从养猪场和零售肉类中培养出的优势菌种，而（）和（）分别是腹泻患者以及零售水果和蔬菜中的优势菌种。所有分离株中约67%为多重耐药（MDR）。（）对氨苄西林和青霉素的耐药性显著高于（）。（）分离株表现出大量异源序列类型（STs），而（）分离株明显分为克隆复合体（CC）CC17和CC94分支。（）分离株主要在住院儿童中检测到，并被鉴定为具有氨苄西林和青霉素耐药性的医院相关CC17分支。值得注意的是，在腹泻患者中检测到的（）ST16和ST65以及（）ST60和ST94也在农场和食品样本中检测到，这表明应对这些STs进行密切监测。在腹泻患者中检测到社区谱系CC94分支，这意味着社区分离株可能进入医院。
本研究强调了对人类健康重要的肠球菌所带来的“同一健康”挑战，以及实施综合预防措施对其进行控制的必要性。
