Canelo E, Phillips O M, del Roure R N
Virology. 1985 Jan 30;140(2):364-7. doi: 10.1016/0042-6822(85)90373-3.
An approach to correlate functions with cistrons in bacteriophage PM2 is presented. Two of the temperature-sensitive mutants obtained differed from the wild type in heat sensitivity and four differed in host range. Comparison of the electrophoretic patterns of DNA from cells infected at the restrictive temperature with those obtained in wildtype infection revealed that viral DNA bands were absent with three additional mutants. Complementation analysis assigned the four host range mutants to cistron I and the three mutants defective in DNA synthesis to cistron IV. Recombination frequencies were used to locate cistrons III and IV on the partial genetic map of PM2.
本文介绍了一种将噬菌体PM2中的功能与顺反子相关联的方法。获得的两个温度敏感突变体在热敏感性方面与野生型不同，四个在宿主范围方面不同。将在限制温度下感染的细胞的DNA电泳图谱与野生型感染中获得的图谱进行比较，发现另外三个突变体没有病毒DNA条带。互补分析将四个宿主范围突变体归为顺反子I，将三个DNA合成有缺陷的突变体归为顺反子IV。重组频率被用于在PM2的部分遗传图谱上定位顺反子III和IV。
